The influence of substrate source on the growth of Ralstonia eutropha, aiming at the production of polyhydroxyalkanoate
The influence of substrate source on the growth of Ralstonia eutropha, aiming at the production of polyhydroxyalkanoate
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以聚羟基脂肪酸酯生产为目的，研究底物来源对真养产碱菌生长的影响
DOI：
10.1590/s0104-66322001000200005
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发表时间：
2001
影响因子：
1.2
通讯作者：
G. Aragão
中科院分区：
文献类型：
--
作者：
C. Marangoni;A. Furigo;G. Aragão
以聚羟基脂肪酸酯为目标，研究了氮源、碳源和温度等培养条件对富养罗尔斯通氏菌（Ralstonia eutropha）生长的影响，并对水解乳糖、转化糖和玉米浆等低成本原料的使用进行了评价。以转化糖为底物时，比生长速率最大（mmax = 0.26 h-1）。两个不同的阶段，在同化的碳源时，水解乳糖的存在，表明同化的第一个葡萄糖，然后半乳糖。为了确认使用半乳糖作为唯一碳源的真养罗尔斯通氏菌的生长，进行了实验，结果表明该细菌能够在该糖的存在下以0.13 h-1的生长速率生长。到目前为止，文献中还没有报道过真养罗尔斯通氏菌使用半乳糖进行生长。玉米浆被认为是一种可行的替代硫酸铵的氮源。在30°C和34°C下进行的实验的结果相似。
With the aim of producing polyhydroxyalkanoates, a study of the influence of culture conditions (nitrogen and carbon sources and temperature) on the growth of Ralstonia eutropha in stirred flasks was carried out and the use of some low-cost sources (hydrolyzed lactose, inverted sugar and corn steep liquor) as evaluated. The best specific growth rate was obtained when inverted sugar was utilized as the substrate (mmax = 0.26 h-1). Two different phases in the assimilation of the carbon source were observed when hydrolyzed lactose was present, suggesting the assimilation first of glucose and then of galactose. To confirm the growth of Ralstonia eutropha using galactose as the only carbon source, experiments were carried out and the results showed that this bacterium is able to grow in the presence of this sugar at a growth rate of 0.13 h-1. The use of galactose by Ralstonia eutropha for its growth has not been reported in the literature until now. Corn steep liquor was found to be a viable alternative nitrogen source to ammonium sulfate. The results of experiments carried out at 30°C and 34°C were similar.