Unraveling the late stages of recombinational repair: Metabolism of DNA junctions in Escherichia coli
Unraveling the late stages of recombinational repair: Metabolism of DNA junctions in Escherichia coli
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DOI：
10.1002/bies.950180911
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发表时间：
1996-09-01
期刊：
影响因子：
4
通讯作者：
Kuzminov, A
中科院分区：
文献类型：
--
作者：
Kuzminov, A
DNA连接是重组修复的副产物，在此过程中，受损的DNA序列在RecA细丝的帮助下侵入完整的同源DNA以形成连接分子。连接是三链或四链的，这取决于有多少DNA单链参与连接分子。在大肠大肠杆菌中，至少有两个独立的途径，以消除连接建议操作。一种是通过RuvAB促进的四链连接的迁移，随后由RuvC解析。在体内，RuvAB和RuvC酶可能在一个单一的复合物中工作，一个resolvasome，从使用过的RecA细丝中清除DNA，并解析四链连接。一种替代的连接去除途径可能是通过RecG促进的三链连接的分支迁移，前提是尚未表征的核酸内切酶活性切割连接分子中的一条链。
DNA junctions are by-products of recombinational repair, during which a damaged DNA sequence, assisted by RecA filament, invades an intact homologous DNA to form a joint molecule. The junctions are three-strand or four-strand depending on how many single DNA strands participate in joint molecules. In E. coli, at least two independent pathways to remove the junctions are proposed to operate. One is via RuvAB-promoted migration of four-strand junctions with their subsequent resolution by RuvC. In vivo, RuvAB and RuvC enzymes might work in a single complex, a resolvasome, to clean DNA from used RecA filaments and to resolve four-strand junctions. An alternative pathway for junction removal could be via RecG-promoted branch migration of three-strand junctions, provided that an as yet uncharacterized endonuclease activity incises one of the strands in the joint molecules.