Human mesenchymal-stem-cells-derived exosomes are important in enhancing porcine islet resistance to hypoxia
Human mesenchymal-stem-cells-derived exosomes are important in enhancing porcine islet resistance to hypoxia
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DOI：
10.1111/xen.12405
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发表时间：
2018-09-01
影响因子：
3.9
通讯作者：
Wang, Wei
中科院分区：
文献类型：
--
作者：
Nie, Wei;Ma, Xiaoqian;Wang, Wei
背景缺氧损伤是胰岛移植物功能障碍的重要因素之一。间充质干细胞（MSCs）由于具有外泌体等旁分泌功能，可用于增强胰岛移植的治疗效果。在这项研究中，我们调查是否从人脐带来源的MSC条件培养基（hu-MSC-CM）的外来体可以增加新生猪胰岛细胞簇（NICCs）暴露于hypoxia.MethodsNeonatal猪胰岛细胞簇的生存和功能培养与hu-MSC-CM，有或没有外来体，和天然培养基RPMI-1640（对照）在缺氧条件下（1%O-2）。结果与RPMI-1640培养液和不含exosomes的hu-MSC-CM培养液相比，含exosomes的hu-MSC-CM培养液中培养的NICCs的存活率、存活率和功能均提高。外泌体在缺氧耐受中起重要作用，提示了改善胰岛移植结果的潜在策略。
BackgroundHypoxia-induced damage is one of the key factors associated with islet graft dysfunction. Mesenchymal stem cells (MSCs) could be used to enhance the therapeutic effect of islet transplantation due to their paracrine potential such as exosomes. In this study, we investigated whether exosomes from human umbilical cord-derived MSC-conditioned medium (hu-MSC-CM) could increase the survival and function of neonatal porcine islet cell clusters (NICCs) exposed to hypoxia.MethodsNeonatal porcine islet cell clusters were cultured with hu-MSC-CM, with or without exosomes, and native medium RPMI-1640 (Control) under hypoxic conditions (1% O-2). The effects of exosomes on NICCs viability and function in vitro were examined by FACS, the Loops system, and the Extracellular Flux assay, respectively.ResultsCompared with NICCs cultured in RPMI-1640 medium and hu-MSC-CM without exosomes, the survival ratio, viability, and function increased in NICCs cultured in hu-MSC-CM with exosomes.ConclusionsThis study found that hu-MSC-CM could protect NICCs from hypoxia-induced dysfunction, and exosomes played an important role in hypoxic resistance, suggesting a potential strategy to improve islet transplantation outcomes.