Genome-wide gene expression changes in genetically stable synthetic and natural wheat allohexaploids
Genome-wide gene expression changes in genetically stable synthetic and natural wheat allohexaploids
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DOI：
10.1111/j.1469-8137.2010.03339.x
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发表时间：
2010-01-01
期刊：
影响因子：
9.4
通讯作者：
Chalhoub, Boulos
中科院分区：
文献类型：
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作者：
Chague, Veronique;Just, Jeremy;Chalhoub, Boulos
本研究旨在了解合成小麦（Triticum aestivum）异源六倍体中基因表达的调控，该异源六倍体由turgidum Triticum AB基因组和tauschii Aegilops D基因组组合而成；我们最近将其定性为基因稳定。我们对基因表达进行了全面的全基因组分析，以表征D基因组祖体细胞变异的影响，代际稳定性以及与天然小麦同种六倍体的比较。我们使用Affymetrix基因芯片小麦基因组阵列，其中有55 049个转录本。结果表明，在合成的异源六倍体中，加性表达代表了大部分的表达调控，其中超过93%的转录本的表达量与亲本RNA混合物中测量到的中间亲本值相等。这就留下了约2000个（约7%）转录本，其中表达是非加性的。没有观察到对任何祖基因组的整体基因表达偏倚或显性，而D基因组祖基因组变异性的高代际稳定性和低影响被揭示。我们的研究表明，小麦异源六倍体的基因表达调控在异源六倍体化早期就建立起来了，并且在世代间高度保守，与天然小麦异源六倍体的表达高度相似。
P>The present study aims to understand regulation of gene expression in synthetic and natural wheat (Triticum aestivum) allohexaploids, that combines the AB genome of Triticum turgidum and the D genome of Aegilops tauschii; and which we have recently characterized as genetically stable.We conducted a comprehensive genome-wide analysis of gene expression that allowed characterization of the effect of variability of the D genome progenitor, the intergenerational stability as well as the comparison with natural wheat allohexaploid. We used the Affymetrix GeneChip Wheat Genome Array, on which 55 049 transcripts are represented.Additive expression was shown to represent the majority of expression regulation in the synthetic allohexaploids, where expression for more than c. 93% of transcripts was equal to the mid-parent value measured from a mixture of parental RNA. This leaves c. 2000 (c. 7%) transcripts, in which expression was nonadditive. No global gene expression bias or dominance towards any of the progenitor genomes was observed whereas high intergenerational stability and low effect of the D genome progenitor variability were revealed.Our study suggests that gene expression regulation in wheat allohexaploids is established early upon allohexaploidization and highly conserved over generations, as demonstrated by the high similarity of expression with natural wheat allohexaploids.