Melatonin pretreatment attenuates 2-bromopropane-induced testicular toxicity in rats
Melatonin pretreatment attenuates 2-bromopropane-induced testicular toxicity in rats
复制标题
褪黑素预处理可减轻 2-溴丙烷诱导的大鼠睾丸毒性
DOI：
10.1016/j.tox.2008.11.005
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发表时间：
2009-02-04
期刊：
影响因子：
4.5
通讯作者：
Ye, Dong-Qing
中科院分区：
文献类型：
--
作者：
Huang, Fen;Ning, Huan;Ye, Dong-Qing
2-溴丙烷（2-BP）被用作臭氧消耗溶剂的替代品，造成雄性工人和实验动物的生殖障碍。最近的一项研究表明，2-BP损害抗氧化细胞防御和增强脂质过氧化（LPO）。褪黑素是一种强大的内源性抗氧化剂。我们假设活性氧（ROS）和脂质过氧化作用参与了2- bp诱导的睾丸毒性。为了验证这一假设，我们研究了褪黑素对2- bp诱导的睾丸毒性的影响。大鼠腹腔注射2-BP (1 g/kg)，加褪黑素（5 mg/kg）或不加褪黑素（5 mg/kg），注射后第7天处死。检查附睾和睾丸组织的生化和组织病理学变化。TUNEL染色和免疫组化检测睾丸组织凋亡细胞活性caspase-3。暴露于2-BP显著降低附睾精子数量和形态正常精子。2-BP还能诱导精小管空泡化、萎缩、精原细胞减少和生殖细胞凋亡。2-BP显著提高血浆和附睾TBARS水平，降低睾丸和附睾GSH含量。褪黑素预处理可抵消2- bp诱导的氧化应激，改善睾丸细胞凋亡，减轻睾丸组织病理损伤。此外，褪黑素预处理可显著减弱2- bp诱导的精子形态变化。我们得出结论，褪黑素预处理通过其活性氧清除和抗凋亡作用减弱2- bp诱导的睾丸毒性。2008爱思唯尔爱尔兰有限公司版权所有。
2-Bromopropane (2-BP) was used as an alternative for ozone-depleting solvents, which caused reproductive disorders in male workers and laboratory animals. A recent study indicated that 2-BP impaired antioxidant cellular defences and enhanced lipid peroxidation (LPO). Melatonin is a powerful endogenous antioxidant. We hypothesized that reactive oxygen species (ROS) and lipid peroxidation are involved in 2-BP-induced testicular toxicities. To test the hypothesis, we investigated the effects of melatonin on 2-BP-induced testicular toxicities. Rats were intraperitoneally injected with 2-BP (1 g/kg) with or without melatonin (5 mg/kg), then sacrificed on 7th day after 2-BP injection. Epididymal and testicular tissues were examined for biochemical and histopathological changes. Apoptotic cells in testis were detected by TUNEL staining and immunohistochemistry for active caspase-3. Exposure to 2-BP significantly decreased epididymal sperm count and morphological normal sperms. 2-BP also induced vacuolation and atrophy of the seminiferous tubules, reduction of spermatogonia and apoptosis of germ cells. 2-BP significantly increased TBARS levels in plasma and epididymis, and decreased GSH content in testis and epididymis. Pretreatment with melatonin counteracted 2-BP-induced oxidative stress, ameliorated apoptosis in testis and attenuated histopathological damage in testis. In addition, pretreatment with melatonin significantly attenuated 2-BP-induced sperm morphological changes. We conclude that pretreatment with melatonin attenuates 2-BP-induced testicular toxicity through its ROS scavenging and anti-apoptotic effects. (C) 2008 Elsevier Ireland Ltd. All rights reserved.