REPLICATION AND TISSUE TROPISM OF THE ALPHAVIRUS SINDBIS IN THE MOSQUITO AEDES-ALBOPICTUS
REPLICATION AND TISSUE TROPISM OF THE ALPHAVIRUS SINDBIS IN THE MOSQUITO AEDES-ALBOPICTUS
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DOI：
10.1006/viro.1995.1447
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发表时间：
1995-09-10
期刊：
影响因子：
3.7
通讯作者：
BROWN, DT
中科院分区：
文献类型：
--
作者：
BOWERS, DF;ABELL, BA;BROWN, DT
我们研究了甲病毒辛德毕斯在白纹伊蚊体内的复制和组织分布。病毒的胃肠外接种导致急性感染伴随着快速病毒复制和持续感染，在此期间，总病毒产量减少。在18天的孵育期内，确定了急性期和持续期病毒RNA合成、病毒产生和感染的器官特异性分布。根据辛德毕斯病毒抗原在特定器官内的发作、峰值和持续时间，将器官分为难治性（卵巢管、马氏管）、清除性（头神经节）、一过性（唾液腺、前中肠、后中肠和胸肌）或持久性（脂肪体-血淋巴、后肠和气管相关细胞）。通过免疫学和超微结构方法鉴定呼吸道组织中的病毒。这代表了甲病毒的一种新向性。这些发现表明，成熟昆虫器官的细胞对病毒感染的反应不同。病毒复制，病毒RNA合成，和器官特异性清除泛嗜性感染之间的相关性进行了观察。我们认为，潜在的时间和空间的动力学特征，这种病毒-无脊椎动物的相互作用可能反映了一种机制，在蚊子宿主中看到的虫媒病毒滴度调制。(C)出版社：Academic Press
We have examined the replication and tissue distribution of the alphavirus Sindbis in the mosquito Aedes albopictus. Parenteral inoculation of virus resulted in an acute infection accompanied by rapid virus replication and a persistent infection, during which total virus production was reduced. Acute and persistent phase virus RNA synthesis, virus production, and organ-specific distribution of infection were determined over an 18-day incubation period. Organs were classified as refractory (ovarioles, Malpighian tubules), cleared (head ganglia), transient (salivary glands, anterior midgut posterior midgut, and thoracic muscle), or persistent (fat bodies-hemolymph, hindgut, and tracheole-associated cells) according to the onset, peak, and duration of Sindbis virus antigens within that particular organ. Virus was identified in respiratory tissue by immunological and ultrastructural methods. This represents a novel tropism for an alphavirus. These findings demonstrate that the cells of mature insect organs respond differently to virus infection. Correlations among virus replication, virus RNA synthesis, and organ-specific clearing of a pantropic infection were observed. We suggest that the underlying temporal and spatial kinetics that characterize this virus-invertebrate interaction may reflect a mechanism for the modulation of the arbovirus titer seen in the mosquito host. (C) 1995 Academic Press, Inc.