Hepatic stellate cells in human schistosomiasis mansoni: A comparative immunohistochemical study with liver
Hepatic stellate cells in human schistosomiasis mansoni: A comparative immunohistochemical study with liver
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DOI：
10.1016/j.actatropica.2005.12.006
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发表时间：
2006-03-01
期刊：
影响因子：
2.7
通讯作者：
Zucoloto, S
中科院分区：
文献类型：
--
作者：
Chang, D;Ramalho, LNZ;Zucoloto, S
本研究比较了血吸虫门静脉周围纤维化和丙型肝炎病毒肝硬化中肝间充质细胞（LMCs）的数量及其增殖活性。采用免疫组化双染色法对40例血吸虫纤维化患者（n =40）、20例丙型肝炎病毒诱导的肝硬化患者（n = 20）和20例正常对照患者的肝活检组织进行增殖细胞核抗原（PCNA）和肌平滑肌肌动蛋白（α - sma）或胶质原纤维酸蛋白（GFAP）检测。研究发现，在血吸虫纤维化中，lmc的数量高于正常肝脏，但低于肝硬化。α - sma和gfap阳性细胞在两种疾病中均增加，但在肝硬化中增加更多。在肝硬化中，α - sma阳性细胞相对于gmap阳性细胞高度占优势。然而，与肝硬化相比，这些细胞在血吸虫病中的比例是相反的。α - sma阳性细胞的PCNA标记指数高于gfap阳性细胞，而对于α - sma或gfap阳性细胞，管状stein纤维化和肝硬化之间没有差异。在血吸虫病中观察到的gfap阳性细胞的优势表明，肝星状细胞（hsc）在人血吸虫肝结缔组织沉积中起主要作用。另一方面，与肝硬化相比，血吸虫纤维化中lmc数量较少可能与血吸虫卵诱导的炎症反应引起的轻度和有限损伤有关。与丙型肝炎病毒产生的分散的坏死-炎症反应相比，曼氏血吸虫卵周围的肉芽肿性炎症似乎动员和激活了减少数量的间充质细胞。(c) 2006 Elsevier B.V.版权所有
This study compares the populations of liver mesenchymal cells (LMCs) and their proliferative activity in schistosomal periportal fibrosis and in hepatitis C virus-induced cirrhosis. LMCs were evaluated by immunohistochemical double staining for proliferating cell nuclear antigen (PCNA) and et-smooth muscle actin (alpha-SMA) or glial fibrillary acid protein (GFAP) in liver biopsies from humans with schistosomal fibrosis (n =40), hepatitis C virus-induced cirrhosis (n = 20), and normal controls (n = 20). The number of LMCs was found to be higher in schistosomal fibrosis than in the normal liver, but lower than in cirrhosis. alpha-SMA- and GFAP-positive cells were increased in both diseases, but more so in cirrhosis. In cirrhotic liver, alpha-SMA-positive cells were highly predominant in relation to GFAP-positive cells. However, there was an inverted ratio between these cells in schistosomiasis as compared to cirrhosis. The PCNA labeling index was higher in alpha-SMA-positive cells than in GFAP-positive cells, and did not differ between pipe-stein fibrosis and liver cirrhosis regarding alpha-SMA- or GFAP-positive cells.The predominance of GFAP-positive cells observed in schistosomiasis suggests that hepatic stellate cells (HSCs) have a major role in connective tissue deposition in the human schistosomal liver. On the other hand, the smaller number of LMCs in schistosomal fibrosis in comparison to liver cirrhosis may be related to mild and limited injury due to the schistosomal egg-induced inflammatory response. The granulomatous inflammation around Schistosoma mansoni eggs appears to mobilize and activate a reduced number of mesenchymal cells in comparison to the scattered necro-inflammatory reaction produced by the hepatitis C virus. (c) 2006 Elsevier B.V. All rights reserved.