Establishment of an animal model of spontaneous cervical lymph node metastasis of laryngeal squamous cell carcinoma and obtaining laryngocarcinoma cells with high metastatic potential
Establishment of an animal model of spontaneous cervical lymph node metastasis of laryngeal squamous cell carcinoma and obtaining laryngocarcinoma cells with high metastatic potential
复制标题
喉鳞状细胞癌自发性颈部淋巴结转移动物模型的建立及高转移性喉癌细胞的获得
DOI：
10.4149/neo_2013_065
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发表时间：
2013-01-01
期刊：
影响因子：
3
通讯作者：
Zhai, S. Q.
中科院分区：
文献类型：
--
作者：
Chen, L. W.;Wang, J. L.;Zhai, S. Q.
为建立喉鳞状细胞癌自发性颈淋巴结转移的动物模型，获得高转移潜能的喉癌细胞，将对数生长期的喉鳞状细胞癌细胞系HEP-2接种于裸鼠舌缘粘膜下。将转移至颈部淋巴结的HEP-2细胞分离、培养并重新接种于裸鼠舌缘粘膜下两次。病理检查证实舌和颈部淋巴结中有肿瘤形成。transwell法检测癌细胞的侵袭和迁移能力。PCR检测到人特异性Alu序列，表明肿瘤细胞来源于人喉鳞癌细胞系HEP-2。最终成功建立了喉鳞癌自发性淋巴结转移动物模型。通过反复接种获得高淋巴结转移潜能的喉鳞状细胞癌细胞。
To establish an animal model of spontaneous cervical lymph node metastasis of laryngeal squamous cell carcinoma and obtain laryngocarcinoma cells with high metastatic potential, laryngeal squamous cell carcinoma cell line HEP-2 in logarithmic phase were inoculated under the lingual margin mucosa of nude mice. HEP-2 cells metastasized to the cervical lymph nodes were isolated, cultured, and re-inoculated under the lingual margin mucosa of nude mice twice. The tumor formation in the tongue and in the cervical lymph nodes was confirmed by pathological examination. Carcinoma cells' ability of invasion and migration was detected by transwell assay. Human specific Alu sequences were detected by PCR, which indicated that the tumor cells originated from human laryngeal squamous cell carcinoma cell line HEP-2. Finally, an animal model of spontaneous lymph node metastasis of laryngeal squamous cell carcinoma was successfully established. Laryngeal squamous cell carcinoma cells with high metastatic potential to lymph nodes were obtained through repeated inoculations.