Global organization of replication time zones of the mouse genome
Global organization of replication time zones of the mouse genome
复制标题
DOI：
10.1101/gr.079566.108
复制
发表时间：
2008-10-01
期刊：
影响因子：
7
通讯作者：
Simon, Itamar
中科院分区：
文献类型：
--
作者：
Farkash-Amar, Shlomit;Lipson, Doron;Simon, Itamar
将基因组划分为不同的复制时区早已被确立。然而，对其组织及其与转录的关系的深入了解并不完整。利用新型同步方法（“婴儿机器”）和基因组 DNA 微阵列，我们首次以高时间分辨率绘制了整个小鼠基因组的复制时间。我们的数据显示，虽然大多数基因组具有不同的复制时间，无论是早期、中期还是晚期 S 期，但基因组的很大一部分是异步复制的。对复制图的分析揭示了复制时区的基因组规模组织。我们发现早期和晚期复制时区之间的基因组区域通常由极大的复制子组成。对复制和转录之间关系的分析表明，早期复制经常与基因的转录潜力相关，而不一定与其实际转录活性相关。这些发现，以及人类和小鼠基因组复制时间之间的强烈保守性，强调了复制时间在转录调控中的重要性。
The division of genomes into distinct replication time zones has long been established. However, an in-depth understanding of their organization and their relationship to transcription is incomplete. Taking advantage of a novel synchronization method ("baby machine") and of genomic DNA microarrays, we have, for the first time, mapped replication times of the entire mouse genome at a high temporal resolution. Our data revealed that although most of the genome has a distinct time of replication either early, middle, or late S phase, a significant portion of the genome is replicated asynchronously. Analysis of the replication map revealed the genomic scale organization of the replication time zones. We found that the genomic regions between early and late replication time zones often consist of extremely large replicons. Analysis of the relationship between replication and transcription revealed that early replication is frequently correlated with the transcription potential of a gene and not necessarily with its actual transcriptional activity. These findings, along with the strong conservation found between replication timing in human and mouse genomes, emphasize the importance of replication timing in transcription regulation.