Regulation of interleukin-21 receptor expression and its signal transduction by WSB-2
Regulation of interleukin-21 receptor expression and its signal transduction by WSB-2
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WSB-2对白细胞介素21受体表达及其信号转导的调控
DOI：
10.1016/j.bbrc.2010.01.004
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Tanaka N and Asao H
中科院分区：
文献类型：
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作者：
Nara H;Onoda T;Rahman M;Araki A;Juliana FM;Tanaka N and Asao H
白介素21(IL-21)是一种调节T细胞、B细胞、NK细胞和髓系细胞功能的多效性细胞因子。IL-21与其同源受体复合体结合，该复合体由IL-21受体(IL-21R)和共同伽马链(γc)受体亚单位组成。我们发现了新的IL-21R结合分子WD-40重复序列，其中含有SoCS-box-2、WSB-2。WSB-2与IL-21R的膜-近端胞浆区域相关，包括box1和box2。WSB-2过表达研究表明，IL-21R表达减少，IL-21诱导的信号转导作用减弱。另一方面，针对WSB-2的小干扰RNA增强了IL-21R的表达水平和IL-21诱导的STAT3的激活，表明WSB-2负调控受体的表达。这份报告首次证明WSB-2是IL-21R表达和IL-21诱导的信号转导的调节因子。
Interleukin-21 (IL-21) is a pleiotropic cytokine that regulates T-cell, B-cell, NK-cell, and myeloid-cell functions. IL-21 binds with its cognate receptor complex, which consists of the IL-21 receptor (IL-21R) and the common gamma chain (γc) receptor subunit. We identified novel IL-21R-binding molecule, WD-40 repeats containing SOCS-box-2, WSB-2. WSB-2 associated with the membrane-proximal intracytoplasmic region of IL-21R, including box1 and box2. Overexpression study of WSB-2 showed the reduction of IL-21R expression and IL-21-induced signal transduction. On the other hand, small interfering RNA for WSB-2 enhanced the expression level of IL-21R and IL-21-induced STAT3 activation, indicating that WSB-2 negatively controls the receptor expression. This report provides the first evidence that WSB-2 is a regulator of IL-21R expression and IL-21-induced signal transduction.