Histopathologic changes of acellularized xenogenic carotid vascular grafts implanted in a pig-to-goat model
Histopathologic changes of acellularized xenogenic carotid vascular grafts implanted in a pig-to-goat model
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DOI：
10.1177/039139880703000107
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发表时间：
2007-01-01
影响因子：
1.7
通讯作者：
Kim, W. G.
中科院分区：
文献类型：
--
作者：
Kim, W. S.;Seo, J. W.;Kim, W. G.
前言：本研究旨在确定脱细胞化小直径异种动脉移植物的体内通畅和再细胞化模式。我们将脱细胞的猪颈动脉移植到山羊的双侧颈动脉中，并在显微镜下分析了这些移植物与受体细胞随时间的再细胞化模式。材料与方法：取重30-40 kg的猪颈动脉，用高渗盐水和十二烷基硫酸钠进行脱细胞。将脱细胞猪颈动脉血管异种移植物（直径0.4 ~ 0.5 cm）制成4 cm长的段，植入10只黑毛山羊的双侧颈动脉。采用彩色多普勒超声定期评价移植的脱细胞异种移植物的体内通畅程度。山羊在预定的时间间隔（植入后1周、1个月、3个月、6个月、12个月）处死，每个间隔2只。检索后，对移植物进行视觉检查和组织病理学检查。为了鉴定平滑肌细胞和功能内皮细胞，还进行了α -平滑肌肌动蛋白和血管性血友病因子的免疫组化染色。结果与结论：所有实验动物均在观察期存活。20例移植物中19例通畅，无血栓形成。显微镜分析显示，从吻合部位开始，移植物完全被宿主的内皮细胞覆盖。移植物逐渐被受体细胞再细胞化，包括成纤维细胞、肌成纤维细胞和平滑肌细胞。综上所述，本研究提示脱细胞异种血管移植是替代小直径血管移植的良好选择。
Introduction: The present study was designed to determine the in vivo patency and recellularization pattern of acellularized small-diameter xenogenic arterial grafts. We implanted acellularized porcine carotid arteries in bilateral carotid arteries of goats and microscopically analyzed the recellularization pattern of these grafts with the recipient's cells over time.Material and Methods: Carotid arteries of pigs weighing 30-40 kg were harvested and decellularized with hypertonic saline followed by sodium dodecyl sulfate. Acellularized porcine carotid vascular xenografts (0.4-0.5 cm in diameter) were prepared into 4 cm-long segments and implanted bilaterally in the carotid arteries of 10 black-haired goats. The in vivo patency of the implanted acellularized xenogenic grafts was evaluated at regular intervals by color Doppler ultrasonography The goats were sacrificed at predetermined intervals (1 week, 1 month, 3 months, 6 months, 12 months after implantation), two animals at each interval. Upon retrieval, visual inspections and histopathologic examinations of the grafts were performed. To identify smooth muscle cells and functioning endothelial cells, immunohistochemical staining for alpha-smooth muscle actin and von Willebrand factor were also performed.Results and Conclusions: All experimental animals survived the observation period. Nineteen out of 20 implanted grafts showed patency with no thrombi. Microscopic analysis revealed that the grafts were completely covered with the hosts' endothelial cells, beginning from anastomotic sites. The grafts were gradually recellularized with recipients'cells including fibroblasts, myofibroblasts and smooth muscle cells. In conclusion, this study suggested that acellularized xenogenic vascular grafts can be a good alternative for the small-diameter vascular graft.