CXCR4 Is Required for Proper Regional and Laminar Distribution of Cortical Somatostatin-, Calretinin-, and Neuropeptide Y-Expressing GABAergic Interneurons
CXCR4 Is Required for Proper Regional and Laminar Distribution of Cortical Somatostatin-, Calretinin-, and Neuropeptide Y-Expressing GABAergic Interneurons
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DOI：
10.1093/cercor/bhq027
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发表时间：
2010-12-01
期刊：
影响因子：
3.7
通讯作者：
Murakami, Fujio
中科院分区：
文献类型：
--
作者：
Tanaka, Daisuke H.;Mikami, Sakae;Murakami, Fujio
皮质 GABA 能中间神经元分为各种亚型，每种亚型都有助于丰富的多样性和精细的抑制细节。尽管每种中间神经元亚型都具有重要的功能，但将它们分类到皮质内适当位置的分子机制仍不清楚。在这里，我们证明趋化因子受体 CXCR4 调节中间神经元亚型的区域和层特异性分布。我们在特定小鼠胚胎发育阶段的中间神经元子集中特异性去除了Cxcr4，并全面分析了成年小鼠9个代表性皮质区域的中间神经元数量及其层状分布。我们发现，在大多数尾内侧和外侧皮质区域以及浅层中，Cxcr4缺失的钙结合蛋白和表达神经肽Y的中间神经元的数量分别减少。此外，Cxcr4缺失的表达生长抑素的中间神经元显示某些皮质区域的浅层数量减少，但其他皮质区域的深层数量减少。这些发现表明，CXCR4 在比之前想象的更广泛的中间神经元亚群中进行适当的区域和层状分布是必需的，并且可能调节某些皮质区域和层中功能性皮质回路的建立。
Cortical GABAergic interneurons are divided into various subtypes, with each subtype contributing to rich variety and fine details of inhibition. Despite the functional importance of each interneuron subtype, the molecular mechanisms that contribute to sorting them to their appropriate positions within the cortex remain unclear. Here, we show that the chemokine receptor CXCR4 regulates the regional and layer-specific distribution of interneuron subtypes. We removed Cxcr4 specifically in a subset of interneurons at a specific mouse embryonic developmental stage and analyzed the number of interneurons and their laminar distribution in 9 representative cortical regions comprehensively in adults. We found that the number of Cxcr4-deleted calretinin- and that of neuropeptide Y-expressing interneurons were reduced in most caudomedial and lateral cortical regions, respectively, and also in superficial layers. In addition, Cxcr4-deleted somatostatin-expressing interneurons showed a reduction in the number of superficial layers in certain cortical regions but of deep layers in others. These findings suggest that CXCR4 is required for proper regional and laminar distribution in a wider interneuron subpopulation than previously thought and may regulate the establishment of functional cortical circuitry in certain cortical regions and layers.