Genetic organization of the HindIII-I region of the single-nucleocapsid nucleopolyhedrovirus of Buzura suppressaria
Genetic organization of the HindIII-I region of the single-nucleocapsid nucleopolyhedrovirus of Buzura suppressaria
复制标题
DOI：
10.1016/s0168-1702(98)00029-x
复制
发表时间：
1998-05-01
期刊：
影响因子：
5
通讯作者：
Vlak, JM
中科院分区：
文献类型：
--
作者：
Hu, ZH;Arif, BM;Vlak, JM
为了研究油桐尺蠖（Buzura suppressaria，BusuNPV）单核衣壳核型多角体病毒（SNPV）的基因组结构，测定了病毒基因组中位于图单位（mu）26.6-29.4的HindIII-I片段。该片段含有两个部分开放阅读框（ORF）和三个完整的开放阅读框（ORF），分别代表AcMNPV ORF 117的同源物多角体包膜蛋白基因（pep）、芋螺毒素样蛋白基因（ctl）、凋亡抑制基因（iap）和超氧化物歧化酶基因（sod）的3'端。这五个基因是首次在SNPV中鉴定出来的。序列分析进一步表明，这些ORF具有与其他杆状病毒同源物相同的保守基序和基因结构。在ctl和lap之间，观察到一个约700个碱基对的基因间区域，其结构类似于DNA复制的非hr起点。BusuNPV HindIII-I片段中ORF的基因组排列与迄今为止苜蓿银纹夜蛾多核衣壳（M）NPV和其他杆状病毒基因组中其同源物的排列非常不同。我们的数据表明，在基因排列的基础上，BusuNPV属于杆状病毒科的一个独特的分类单元，证实了我们以前的结论来自几个BusuNPV基因的遗传分析。(C)1998 Elsevier Science B. V.保留所有权利。
In order to investigate the genomic organization of the single-nucleocapsid nucleopolyhedrovirus (SNPV) of Buzura suppressaria (BusuNPV), the HindIII-I fragment located at map units (mu) 26.6-29.4 of the viral genome was sequenced. The fragment contained two partial and three complete open reading frames (ORFs) representing the 3' end of a polyhedron envelope protein gene (pep), a homologue of the AcMNPV ORF117, a conotoxin-like protein gene (ctl), an inhibitor of apoptosis gene (iap) and a superoxide dismutase gene (sod), respectively. These five genes were identified for the first time in a SNPV. Sequence analysis further revealed that these ORFs have the same conserved motifs and gene structure as those observed in their homologues from other baculoviruses. Between ctl and lap, an intergenic region of about 700 basepairs with structure similar to non-hr origins of DNA replication was observed. The genomic arrangement of the ORFs in the BusuNPV HindIII-I fragment is very different from the arrangement of their homologues in the genome of Autographa californica multiple nucleocapsid (M) NPV and other baculoviruses to date. Our data suggest that on the basis Of gene arrangement, BusuNPV belongs to a distinct taxon within the Baculoviridae family, corroborating our previous conclusions derived from phylogeny analysis of several BusuNPV genes. (C) 1998 Elsevier Science B.V. All rights reserved.