Distribution of estrogen receptor-β messenger ribonucleic acid in the male sheep hypothalamus
Distribution of estrogen receptor-β messenger ribonucleic acid in the male sheep hypothalamus
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DOI：
10.1095/biolreprod60.6.1279
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发表时间：
1999-06-01
影响因子：
3.6
通讯作者：
Jackson, GL
中科院分区：
文献类型：
--
作者：
Hileman, SM;Handa, RJ;Jackson, GL
作为确定新发现的雌激素受体(ER)-β对生殖可能影响的第一步，我们使用原位杂交和大鼠ER-P cRNA探针定位了ER-β在雄性绵羊下丘脑中的mRNA。在视前区(POA)、终纹床核、室旁核和视上核中杂交信号最强。在交叉后区(RCH)、下丘脑前区、背内侧下丘脑和下丘脑外侧区观察到相对适量的杂交信号。仅在下丘脑腹内侧核、视交叉上核和弓状核观察到低水平的杂交信号。在雄性绵羊下丘脑的几个区域中存在ER-βmRNA，这表明该受体具有多种功能。ER-β在绵羊下丘脑中的分布与对大鼠的描述相似，表明在不同物种之间具有高度的功能保守。ER-β在控制生殖的下丘脑的POA和RCH中的存在表明了它在影响生殖中的作用。
As a first step in determining possible influences of the newly discovered estrogen receptor (ER)-beta on reproduction, we have localized mRNA for ER-beta within the male sheep hypothalamus using in situ hybridization and a rat ER-P cRNA probe. Highest amounts of hybridization signal were observed in the preoptic area (POA), bed nucleus of the stria terminalis, paraventricular nucleus, and supraoptic nucleus. Relatively moderate amounts of hybridization signal were observed in the retrochiasmatic area (RCH), anterior hypothalamic area, dorsomedial hypothalamus, and lateral hypothalamus. Only a low level of hybridization signal was observed in the ventromedial hypothalamus, suprachiasmatic nucleus, and arcuate nucleus. The presence of ER-beta mRNA in several areas of the male sheep hypothalamus suggests multiple functions for this receptor. The distribution of ER-beta in the ovine hypothalamus was similar to that described for the rat, suggesting a high degree of functional conservation across species. A role for ER-beta in influencing reproduction is suggested by its presence in the POA and RCH, regions of the hypothalamus that control reproduction.