Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: A possible role for SirT3 activation
Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: A possible role for SirT3 activation
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DOI：
10.1016/j.mito.2013.09.002
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发表时间：
2013-11-01
期刊：
影响因子：
4.4
通讯作者：
Palmeira, Carlos Marques
中科院分区：
文献类型：
--
作者：
Teodoro, Joao Soeiro;Duarte, Filipe Valente;Palmeira, Carlos Marques
黄连素是一种具有抗糖尿病作用的异喹啉生物碱。尽管肝脏和肝脏线粒体在全身代谢中起着中心作用，但在肥胖模型中，黄连素对肝脏线粒体功能的影响仍不清楚。在这里，我们证明，黄连素治疗恢复线粒体效率时，改变了高脂肪饲料。从高脂饲养的大鼠肝脏分离的线粒体表现为积累钙的能力下降和氧化磷酸化(OXPHOS)能力下降，表现为线粒体膜电位、氧耗和细胞ATP水平的下降。有趣的是，黄连素对线粒体功能的恢复与线粒体sirtuin 3(SirT3)活性的增加有关。结论：黄连素对代谢综合征的有效保护作用可能依赖于增加线粒体SirT3的活性，使线粒体功能正常化，以及防止由于OXPHOS受损而导致的能量缺乏状态。(C)2013年爱思唯尔公司和线粒体研究会。版权所有。
Berberine is an isoquinoline alkaloid with anti-diabetic properties. Despite the central role of liver and thus hepatic mitochondria in whole-body metabolism, berberine effects on hepatic mitochondrial function in an obesity model are still unknown. Here, we demonstrate that berberine treatment recovers mitochondrial efficiency when altered by a high-fat feeding. Mitochondria isolated from the liver of high-fat fed rats exhibited decreased capacity to accumulate calcium and impaired oxidative phosphorylation (OXPHOS) capacity, as shown by impaired mitochondrial membrane potential, oxygen consumption and cellular ATP levels. Interestingly, the recovery of mitochondrial function by berberine was associated with an increased activity of the mitochondrial sirtuin 3 (SirT3). In conclusion, berberine potent protective effects against metabolic syndrome may rely on increasing mitochondrial SirT3 activity, normalizing mitochondrial function and preventing a state of energetic deficit caused by impaired OXPHOS. (C) 2013 Elsevier B.V. and Mitochondria Research Society. All rights reserved.