Anti-epithelial cell adhesion molecule antibodies and the detection of circulating normal-like breast tumor cells.
Anti-epithelial cell adhesion molecule antibodies and the detection of circulating normal-like breast tumor cells.
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DOI：
10.1093/jnci/djn419
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发表时间：
2009-01-07
影响因子：
10.3
通讯作者：
Foekens, John A.
中科院分区：
文献类型：
--
作者：
Sieuwerts, Anieta M.;Kraan, Jaco;Bolt, Joan;van der Spoel, Petra;Elstrodt, Fons;Schutte, Mieke;Martens, John W. M.;Gratama, Jan-Willem;Sleijfer, Stefan;Foekens, John A.
识别癌症患者外周血中特定亚型的循环肿瘤细胞可以提供有关转移生物学的信息，并改善患者的管理。然而，为了有效，用于识别循环肿瘤细胞的方法必须检测所有肿瘤细胞类型。我们调查了全球基因表达谱定义的五种人类乳腺癌细胞亚型-正常样、基础、HER2阳性以及腔A和B-是否被美国食品和药物管理局批准的CellSearch识别，该测试使用针对细胞表面表达的上皮细胞黏附分子(EpCAM)的抗体来分离循环中的肿瘤细胞。我们使用全球基因表达谱来确定34个人乳腺癌细胞系(15个腔状，9个正常样，5个基底样，5个Her2阳性)的亚型。我们将其中10个细胞系的50-150个细胞与一名健康人体捐赠者的7.5mL血液混合，并进行CellSearch试验以分离乳腺癌细胞。我们发现，CellSearch分离方法使用循环肿瘤细胞表面的EpCAM进行细胞分离，尤其不能识别通常具有侵袭性特征的正常样乳腺癌细胞。需要新的测试，包括特异性识别正常乳腺肿瘤细胞但不识别造血细胞的抗体。
Identification of specific subtypes of circulating tumor cells in peripheral blood of cancer patients can provide information about the biology of metastasis and improve patient management. However, to be effective, the method used to identify circulating tumor cells must detect all tumor cell types. We investigated whether the five subtypes of human breast cancer cells that have been defined by global gene expression profiling—normal-like, basal, HER2-positive, and luminal A and B—were identified by CellSearch, a US Food and Drug Administration–approved test that uses antibodies against the cell surface–expressed epithelial cell adhesion molecule (EpCAM) to isolate circulating tumor cells. We used global gene expression profiling to determine the subtypes of a well-defined panel of 34 human breast cancer cell lines (15 luminal, nine normal-like, five basal-like, and five Her2-positive). We mixed 50-150 cells from 10 of these cell lines with 7.5 mL of blood from a single healthy human donor, and the mixtures were subjected to the CellSearch test to isolate the breast cancer cells. We found that the CellSearch isolation method, which uses EpCAM on the surface of circulating tumor cells for cell isolation, did not recognize, in particular, normal-like breast cancer cells, which in general have aggressive features. New tests that include antibodies that specifically recognize normal-like breast tumor cells but not cells of hematopoietic origin are needed.
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影响因子：
45.3
作者：
Cristofanilli, M;Hayes, DF;Terstappen, LWMM
通讯作者：
Terstappen, LWMM
影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
影响因子：
50.5
作者：
Nole, F.;Munzone, E.;Sandri, M. T.
通讯作者：
Sandri, M. T.
影响因子：
11.2
作者：
Smid, Marcel;Wang, Yixin;Martens, John W. M.
通讯作者：
Martens, John W. M.
影响因子：
64.5
作者：
Yang, J;Mani, SA;Weinberg, RA
通讯作者：
Weinberg, RA