Proteomic analysis of the oxidative stress responses in Candida albicans
Proteomic analysis of the oxidative stress responses in Candida albicans
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DOI：
10.1002/pmic.200600575
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发表时间：
2007-03-01
期刊：
影响因子：
3.4
通讯作者：
Hecker, Michael
中科院分区：
文献类型：
--
作者：
Kusch, Harald;Engelmann, Susanne;Hecker, Michael
一个有效的氧化应激反应（OSR）是兼性致病性白色念珠菌在人类宿主内生存的重要条件。我们采用大规模二维蛋白凝胶电泳方法分析了双氧水和硫醇氧化剂二胺处理后白色念珠菌的应激反应机制。用放射性L-[S-35]-蛋氨酸对蛋白质进行脉冲标记后，对体内蛋白质合成进行定量，结果显示过氧化氢和二胺的蛋白质组特征，两种应激源的21个上调蛋白显著重叠。在这些诱导蛋白中，有已知具有抗氧化功能的酶，如过氧化氢酶或硫氧还蛋白还原酶和一组氧化还原酶。对CAP1基因突变体的二维凝胶分析显示，氧化应激调节因子Cap1p控制了12种蛋白的合成。这12种蛋白均在过氧化氢或二胺氧化刺激后被诱导，这也说明了其在白色念珠菌OSR中的重要性。
An efficient oxidative stress response (OSR) is important for the facultative pathogenic yeast Candida albicans to survive within the human host. We used a large scale 2-D protein gel electrophoresis approach to analyze the stress response mechanisms of C. albicans after treatment with hydrogen peroxide and the thiol oxidizing agent, diamide. Quantitation of in vivo protein synthesis after pulse labeling of the proteins with radioactive L-[S-35]-methionine resulted in characteristic proteome signatures for hydrogen peroxide and diamide with significant overlap of 21 up-regulated proteins for both stressors. Among the induced proteins were enzymes with known antioxidant functions like catalase or thioredoxin reductase and a set of oxidoreductases. 2-D gel analysis of mutants in the CAP1 gene revealed that the synthesis of 12 proteins is controlled by the oxidative stress regulator Cap1p. Stressing its importance for the C. albicans OSR, all 12 proteins were also induced after oxidative challenge by hydrogen peroxide or diamide.