Three-dimensional paper-based electrochemiluminescence immunodevice for multiplexed measurement of biomarkers and point-of-care testing
Three-dimensional paper-based electrochemiluminescence immunodevice for multiplexed measurement of biomarkers and point-of-care testing
复制标题
用于生物标志物多重测量和即时检测的三维纸基电化学发光免疫装置
DOI：
10.1016/j.biomaterials.2011.10.065
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发表时间：
2012-02-01
期刊：
影响因子：
14
通讯作者：
Yu, Jinghua
中科院分区：
文献类型：
--
作者：
Ge, Lei;Yan, Jixian;Yu, Jinghua
在这项工作中，电化学发光（ECL）免疫分析首次被引入到最近提出的基于纸张的微流控分析装置（μPADs）中，该装置基于直接在纸上丝网印刷电极。与商业化电极相比，丝网印刷纸电极对于这种基于纸张的ECL装置在简单、低成本和一次性应用方面的进一步发展将更为重要。为了在μPAD上进一步进行高性能、高通量、简单且廉价的ECL免疫分析以用于即时检测，首次展示了一种蜡印三维（3D）基于纸张的ECL装置。在这种3D基于纸张的ECL装置中，八个碳工作电极及其导电焊盘被丝网印刷在一张正方形纸上，并且在堆叠后与另一张正方形纸上的相同的Ag/AgCl参比电极和碳对电极共用。使用典型的三联吡啶钌（II） - 三正丙胺ECL系统，通过对实际临床血清样本中的四种肿瘤标志物进行诊断，对这种3D基于纸张的ECL装置进行了应用测试。借助于一个简易的装置支架和安装在分析仪上的一个部分切换装置，八个工作电极被依次接入电路，在室温下于0.5到1.1 V的扫描范围内触发ECL反应。此外，这种3D基于纸张的ECL装置可以很容易地与最近出现的纸电子器件集成和组合，以进一步开发简单、灵敏、低成本、一次性和便携式的μRAD，用于即时检测、偏远地区的公共卫生和环境监测，无论是发展中国家还是发达国家。（C）2011 Elsevier Ltd. 保留所有权利。
In this work, electrochemiluminescence (ECL) immunoassay was introduced into the recently proposed microfluidic paper-based analytical device (mu PADs) based on directly screen-printed electrodes on paper for the very first time. The screen-printed paper-electrodes will be more important for further development of this paper-based ECL device in simple, low-cost and disposable application than commercialized ones. To further perform high-performance, high-throughput, simple and inexpensive ECL immunoassay on mu PAD for point-of-care testing, a wax-patterned three-dimensional (3D) paper-based ECL device was demonstrated for the very first time. In this 3D paper-based ECL device, eight carbon working electrodes including their conductive pads were screen-printed on a piece of square paper and shared the same Ag/AgCl reference and carbon counter electrodes on another piece of square paper after stacking. Using typical tris-(bipyridine)-ruthenium (II) - tri-n-propylamine ECL system, the application test of this 3D paper-based ECL device was performed through the diagnosis of four tumor markers in real clinical serum samples. With the aid of a facile device-holder and a section-switch assembled on the analyzer, eight working electrodes were sequentially placed into the circuit to trigger the ECL reaction in the sweeping range from 0.5 to 1.1 V at room temperature. In addition, this 3D paper-based ECL device can be easily integrated and combined with the recently emerging paper electronics to further develop simple, sensitive, low-cost, disposable and portable mu RAD for point-of-care testing, public health and environmental monitoring in remote regions, developing or developed countries. (C) 2011 Elsevier Ltd. All rights reserved.