Higher expression of oncoproteins c-myc, c-erbB-2/neu, PCNA, and p53 in metastasizing colorectal cancer than in nonmetastasizing tumors
Higher expression of oncoproteins c-myc, c-erbB-2/neu, PCNA, and p53 in metastasizing colorectal cancer than in nonmetastasizing tumors
复制标题
DOI：
10.1007/bf02306092
复制
发表时间：
1996-11-01
影响因子：
3.7
通讯作者：
Crowe, PJ
中科院分区：
文献类型：
--
作者：
Yang, JL;Ow, KT;Crowe, PJ
背景：单个癌基因的表达可以预测转移性结直肠癌（CRC）患者的预后。方法：采用免疫组化法检测34例大肠癌转移灶、25例大肠癌肝转移灶及25例未转移灶癌基因c-myc、c-erbB-2/neu（c-neu）、PCNA和p53的表达。转移性原发性结直肠癌表达增殖细胞核抗原（PCNA），c-neu和c-myc的水平显着高于非转移性原发性癌。p53在转移组中的表达也高于非转移组，但差异无统计学意义。所有这些标志物的表达频率在同一患者的转移性原发性CRC和肝继发性CRC中相似。单个标记物的表达与组织学分级、DNA倍体、局部复发、肺转移和生存率无关。然而，当两组一起评估时，c-myc阳性表达更可能发生在低分化肿瘤中，而PCNA表达随着Dukes分期的进展而增加。这些结果表明，c-myc，c-neu，PCNA和p53的过度表达可能发生在大肠癌，可能转移到肝脏。
Background: Expression of individual oncogenes may predict outcome in patients with metastatic colorectal cancer (CRC). We studied the oncogene profile in the tumors of patients with CRC and assessed their value as predictors of liver metastases,Methods: The oncoproteins c-myc, c-erbB-2/neu (c-neu), PCNA and p53, were measured by immunohistochemistry in sections of metastasizing human CRC (n=34) and their liver secondaries as well as in sections of nonmetastasizing CRC (n=25).Results: The metastasizing primary CRC expressed proliferating-cell nuclear antigen (PCNA), c-neu, and c-myc at significantly higher levels than the nonmetastasizing primary cancer. p53 was also overexpressed in the metastatic group compared with the nonmetastasizing CRC, but this difference was not significant. The frequency of expression of all these markers was similar in the metastasizing primary CRC and the liver secondaries from the same patients. There was no correlation between the expression of the individual markers and histological grade, DNA ploidy, and subsequent local recurrence and lung metastasis and survival, However, when both groups were assessed together, positive expression of c-myc was more likely to occur in poorly differentiated tumors, whereas PCNA expression increased with more advanced Dukes stages.Conclusion: These results suggest that the overexpression of c-myc, c-neu, PCNA, and p53 may occur in CRC that are likely to metastasise to the liver.