Structural and genomic properties of the hyperthermophilic archaeal virus ATV with an extracellular stage of the reproductive cycle
Structural and genomic properties of the hyperthermophilic archaeal virus ATV with an extracellular stage of the reproductive cycle
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DOI：
10.1016/j.jmb.2006.04.027
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发表时间：
2006-06-23
影响因子：
5.6
通讯作者：
Garrett, Roger A.
中科院分区：
文献类型：
--
作者：
Prangishvili, David;Vestergaard, Gisle;Garrett, Roger A.
嗜热古生菌属Acidianus的一种新病毒ATV具有在宿主细胞外和独立于宿主细胞进行主要形态发育的独特性质。病毒粒子作为柠檬形状的无尾粒子从宿主细胞中挤出，之后在接近自然栖息地85摄氏度的温度下，它们在每一尖端形成长尾。细胞外发育的尾巴构成管子，这些管状结构终止于无尾粒子中没有观察到的锚状结构。管内有一根细丝，呈周期性结构。尾巴发育导致病毒粒子体积减少一半，同时病毒粒子表面略有扩张。这个圆形的双链DNA基因组包含62,730个核苷酸，对于隐翅虫病毒来说是个例外，因为它携带四个假定的转座元件以及基因，而这些基因以前只与古生菌的可自我传播的质粒有关。共编码72种预测蛋白，其中11种结构蛋白的相对分子质量在12-90 kDa之间。一些较大的蛋白质富含卷曲卷曲和/或低复杂性的序列结构域，这在古生菌中是不常见的。一种蛋白质，特别是P800，在结构特性上类似于中间丝蛋白质。它在双尾病毒粒子中被修饰，但在无尾病毒粒子中不被修饰，它可能有助于病毒尾巴的发展。对于隐形杆菌病毒来说，感染ATV会导致病毒复制和随后的细胞裂解，或者导致受感染的细胞转化为溶原体。溶源循环涉及病毒基因组与宿主染色体的整合，可能是由病毒编码的整合酶促进的，这一过程可以被不同的胁迫因素中断。(C)2006爱思唯尔有限公司。保留所有权利。
A novel virus, ATV, of the hyperthermophilic archaeal genus Acidianus has the unique property of undergoing a major morphological development outside of, and independently of, the host cell. Virions are extruded from host cells as lemon-shaped tail-less particles, after which they develop long tails at each pointed end, at temperatures close to that of the natural habitat, 85 degrees C. The extracellularly developed tails constitute tubes, which terminate in an anchor-like structure that is not observed in the tail-less particles. A thin filament is located within the tube, which exhibits a periodic structure. Tail development produces a one half reduction in the volume of the virion, concurrent with a slight expansion of the virion surface. The circular, double-stranded DNA genome contains 62,730 bp and is exceptional for a crenarchaeal virus in that it carries four putative transposable elements as well as genes, which previously have been associated only with archaeal self-transmissable plasmids. In total, it encodes 72 predicted proteins, including 11 structural proteins with molecular masses in the range of 12 to 90 kDa. Several of the larger proteins are rich in coiled coil and/or low complexity sequence domains, which are unusual for archaea. One protein, in particular P800, resembles an intermediate filament protein in its structural properties. It is modified in the two-tailed, but not in the tail-less, virion particles and it may contribute to viral tail development. Exceptionally for a crenarchaeal virus, infection with ATV results either in viral replication and subsequent cell lysis or in conversion of the infected cell to a lysogen. The lysogenic cycle involves integration of the viral genome into the host chromosome, probably facilitated by the virus-encoded integrase and this process can be interrupted by different stress factors. (c) 2006 Elsevier Ltd. All rights reserved.