Involvement of reactive oxygen species during early stages of ectomycorrhiza establishment between Castanea sativa and Pisolithus tinctorius
Involvement of reactive oxygen species during early stages of ectomycorrhiza establishment between Castanea sativa and Pisolithus tinctorius
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DOI：
10.1007/s00572-006-0091-4
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发表时间：
2007-05-01
期刊：
影响因子：
3.9
通讯作者：
Lino-Neto, Teresa
中科院分区：
文献类型：
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作者：
Baptista, Paula;Martins, Anabela;Lino-Neto, Teresa
活性氧（ROS）和抗氧化系统参与外生菌根（ECM）建立的证据是缺乏的。在本文中，我们评估了活性氧的产生和超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性在早期接触的ECM真菌Pisolithus tinctorius与板栗（板栗树）的根。将根与P. tinctorius菌丝体接触，并通过测定真菌接触早期阶段的H2 O2和O-2（中心点-）水平来评估ROS产生。检测到H2 O2产生的三个峰，前两个峰与O-2（中心点）爆发一致。第一个H2 O2产生高峰与SOD活性的增加相吻合，而CAT活性似乎与H2 O2清除有关。在着色假单胞菌诱导的C.在真菌接触的早期阶段制备的sativa粗提取物。差异菌丝生长相匹配的H2 O2生产曲线与延迟进行了检测。结果表明，在ECM建立的早期阶段，H2 O2的结果从ROS清除酶的抑制，并在共生体建立过程中的信号中发挥作用。
Evidence for the participation of reactive oxygen species (ROS) and antioxidant systems in ectomycorrhizal (ECM) establishment is lacking. In this paper, we evaluated ROS production and the activities of superoxide dismutase (SOD) and catalase (CAT) during the early contact of the ECM fungus Pisolithus tinctorius with the roots of Castanea sativa (chestnut tree). Roots were placed in contact with P. tinctorius mycelia, and ROS production was evaluated by determining the levels of H2O2 and O-2(center dot-) during the early stages of fungal contact. Three peaks of H2O2 production were detected, the first two coinciding with O-2(center dot-) bursts. The first H2O2 production peak coincided with an increase in SOD activity, whereas CAT activity seemed to be implicated in H2O2 scavenging. P. tinctorius growth was evaluated in the presence of P. tinctorius-elicited C. sativa crude extracts prepared during the early stages of fungal contact. Differential hyphal growth that matched the H2O2 production profile with a delay was detected. The result suggests that during the early stages of ECM establishment, H2O2 results from an inhibition of ROS-scavenging enzymes and plays a role in signalling during symbiotic establishment.