Outer membrane lipoprotein VacJ is required for the membrane integrity, serum resistance and biofilm formation of Actinobacillus pleuropneumoniae
Outer membrane lipoprotein VacJ is required for the membrane integrity, serum resistance and biofilm formation of Actinobacillus pleuropneumoniae
复制标题
外膜脂蛋白 VacJ 是胸膜肺炎放线杆菌膜完整性、血清抗性和生物膜形成所必需的
DOI：
10.1016/j.vetmic.2015.11.021
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发表时间：
2016-02-01
影响因子：
3.3
通讯作者：
Wang, Chunlai
中科院分区：
文献类型：
--
作者：
Xie, Fang;Li, Gang;Wang, Chunlai
胸膜肺炎放线杆菌的外膜蛋白是感染的介质，作为宿主防御系统的靶标。外膜脂蛋白VacJ参与几种病原菌的血清抵抗和细胞间扩散。为研究VacJ在胸膜肺炎放线杆菌致病性中的作用，构建了VacJ基因缺失突变株MD12 Δ vacJ。MD12 Delta vacJ突变体对KCl、SDS + EDTA和几种抗生素的敏感性增加，表明vacJ基因突变导致外膜的稳定性受损。MD12 Δ vacJ突变体中增加的NPN荧光和显著的细胞形态学变化进一步证明了VacJ脂蛋白在维持A.胸膜肺炎此外，与野生型菌株相比，MD12 Delta vacJ突变体表现出降低的血清存活率和补体杀伤。有趣的是，与野生型菌株相比，MD12 Delta vacJ突变体显示出减少的生物膜形成。据我们所知，这是VacJ脂蛋白有助于细菌生物膜形成的第一个描述。本研究中的数据说明了VacJ脂蛋白在维持细胞完整性、血清抵抗力和A.胸膜肺炎(C)2015 Elsevier B.V.版权所有。
The outer membrane proteins of Actinobacillus pleuropneumoniae are mediators of infection, acting as targets for the host's defense system. The outer membrane lipoprotein VacJ is involved in serum resistance and intercellular spreading in several pathogenic bacteria. To investigate the role of VacJ in the pathogenicity of Actinobacillus pleuropneumoniae, the vacJ gene-deletion mutant MD12 Delta vacJ was constructed. The increased susceptibility to KCl, SDS plus EDTA, and several antibiotics in the MD12 Delta vacJ mutant suggested that the stability of the outer membrane was impaired as a result of the mutation in the vacJ gene. The increased NPN fluorescence and significant cellular morphological variation in the MD12 Delta vacJ mutant further demonstrated the crucial role of the VacJ lipoprotein in maintaining the outer membrane integrity of A. pleuropneumoniae. In addition, the MD12 Delta vacJ mutant exhibited decreased survival from the serum and complement killing compared to the wild-type strain. Interestingly, the MD12 Delta vacJ mutant showed reduced biofilm formation compared to the wild-type strain. To our knowledge, this is the first description of the VacJ lipoprotein contributing to bacterial biofilm formation. The data presented in this study illustrate the important role of the VacJ lipoprotein in the maintenance of cellular integrity, serum resistance, and biofilm formation in A. pleuropneumoniae. (C) 2015 Elsevier B.V. All rights reserved.