Large-scale morphological survey of mouse retinal ganglion cells
Large-scale morphological survey of mouse retinal ganglion cells
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DOI：
10.1002/cne.10323
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发表时间：
2002-09-16
影响因子：
2.5
通讯作者：
He, SG
中科院分区：
文献类型：
--
作者：
Sun, WZ;Li, N;He, SG
使用“DiOlistic”方法（Gan等人，[2000] Neuron 27：219-225）标记小鼠视网膜的分离的整装制备物中的520个神经节细胞，并根据它们的形态学性质进行分类。使用基因枪将涂覆有亲脂性染料（DiI）的钨颗粒推进到整装视网膜中。当染料涂覆的颗粒接触细胞膜时，整个细胞被标记。神经节细胞根据其索马大小、树突野大小、分层模式和水平分为四组。将广泛的单层细胞分为三组：RG（A）细胞（大索马，大树突状野）、RG（B）细胞（小至中等大小的索马，小至中等大小的树突状野）和RG（C）细胞（小至中等大小的索马，中等大小至大树突状野）。将双层细胞分类为RG（D）。这项研究代表了迄今为止最完整的小鼠视网膜神经节细胞的形态学分类，并为进一步了解生理学和形态学以及神经节细胞功能与遗传操作动物的相关性提供了基础。
Five hundred twenty ganglion cells in an isolated whole-mount preparation of the mouse retina were labeled using the "DiOlistic" method (Gan et al. [2000] Neuron 27:219-225) and were classified according to their morphological properties. Tungsten particles coated with a lipophilic dye (DiI) were propelled into the whole-mount retina using a gene gun. When a dye-coated particle contacted the cell membrane, the entire cell was labeled. The ganglion cells were classified into four groups based on their soma size, dendritic field size, and pattern and level of stratification. Broadly monostratified cells were classified into three groups: RG(A) cells (large soma, large dendritic field), RG(B) cells (small to medium-sized soma, small to medium-sized dendritic field), and RG(C) cells (small to medium-sized size soma, medium-sized to large dendritic field). Bistratified cells were classified as RG(D). This study represents the most complete morphological classification of mouse retinal ganglion cells available to date and provides a foundation for further understanding of the correlation of physiology and morphology and ganglion cell function with genetically manipulated animals.