In situ expression of 15 kDa interferon alpha responsive gene in the developing tooth germ of the mouse lower first molar
In situ expression of 15 kDa interferon alpha responsive gene in the developing tooth germ of the mouse lower first molar
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DOI：
10.1007/s10735-010-9277-3
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发表时间：
2010-10-01
影响因子：
3.2
通讯作者：
Sakai, Hidetaka
中科院分区：
文献类型：
--
作者：
Akhter, Merina;Kobayashi, Ieyoshi;Sakai, Hidetaka
我们先前进行了cDNA消减小鼠下颌骨在胚胎第10.5天（E10.5）和E12.0，使调控基因的牙齿发生的配置文件。15kDa干扰素α反应基因（Ifrg15）是E12.0下颌骨中高表达的基因之一。本研究通过原位杂交检测小鼠下颌第一磨牙中Ifrg15 mRNA的精确表达模式，以评估该基因在牙发生中可能的功能作用。E10.5和E12.0时，Ifrg15 mRNA在下颌骨的上皮和间充质组织中表达。Ifrg15原位信号在E14.0时在上皮芽和周围间充质中检测到，并且在E15.0时存在于成釉器官（包括初级釉结）和下层间充质中。E16.0时，原位信号仅限于内、外釉上皮和中间层。在E17.0和E18.0，Ifrg15 mRNA的信号进一步局限于内釉上皮和相邻的中间层。因此，Ifrg15 mRNA的表达定位于成釉细胞和成牙本质细胞在出生后1.0至3.0天。然而，原位信号明显弱于胚胎期。Ifrg15 mRNA在牙胚和颅面各器官中均有表达。这些结果表明，IFRG 15与牙的发生，特别是成釉细胞和成牙本质细胞的分化，以及颅面器官的形态发生密切相关。
We previously performed cDNA subtraction between the mouse mandibles at embryonic day 10.5 (E10.5) and E12.0 to make a profile of the regulator genes for odontogenesis. Fifteen kDa interferon alpha responsive gene (Ifrg15) is one of several highly-expressed genes in the E12.0 mandible. The current study examined the precise expression patterns of Ifrg15 mRNA in the mouse mandibular first molar by in situ hybridization to evaluate the possible functional roles of this gene in odontogenesis. Ifrg15 mRNA was expressed in the epithelial and mesenchymal tissues of the mandible at E10.5 and E12.0. The Ifrg15 in situ signal was detected in the epithelial bud and the surrounding mesenchyme at E14.0, and was present in the enamel organ including the primary enamel knot, and in the underlying mesenchyme at E15.0. The in situ signal was restricted in the inner and outer enamel epithelia and the stratum intermedium at E16.0. The signal of Ifrg15 mRNA was further restricted to the inner enamel epithelium and the adjacent stratum intermedium at E17.0 and E18.0. Consequently, the expression of Ifrg15 mRNA was localized in the ameloblasts and odontoblasts at postnatal days 1.0 to 3.0. However, the in situ signal was markedly weaker than at the embryonic period. The expression of Ifrg15 mRNA was coincidently observed in various craniofacial organs as well as in the tooth germ. These results suggest that Ifrg15 is closely related to odontogenesis, especially the differentiation of the ameloblasts and odontoblasts, and to the morphogenesis of the craniofacial organs.