Genetic mapping of anti-apoptosis pathways in myeloid progenitor cells.
Genetic mapping of anti-apoptosis pathways in myeloid progenitor cells.
复制标题
骨髓祖细胞抗凋亡途径的基因图谱。
DOI：
10.1007/978-1-60327-017-5_20
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Songyang,Zhou
中科院分区：
文献类型：
--
作者：
Liu,Dan;Songyang,Zhou
In mammalian cells, apoptotic and anti-apoptotic pathways may be investigated using a variety of biochemical, molecular, and genetic approaches. Retrovirus mediated genetic screens have proven a powerful tool in mapping out the network of players in a number of signaling pathways. We have developed the ERM (for enhanced retroviral mutagen) mutagenesis approach to identify novel players in the growth factor dependent survival pathways. ERM has been shown to be efficient and amenable to genome wide genetic screens in mammalian cells without the need of cDNA library construction. The advantages of the ERM method include regulatable expression, flexible design, and efficiency.
