Diversity and distribution of bacterioplankton in the coastal upwelling waters off Hainan Island, China
Diversity and distribution of bacterioplankton in the coastal upwelling waters off Hainan Island, China
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DOI：
10.1007/s13131-021-1807-1
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发表时间：
2022-03-01
影响因子：
1.4
通讯作者：
Gong, Jun
中科院分区：
文献类型：
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作者：
Gong, Fahui;Ji, Qixing;Gong, Jun
2016年8月，采用高通量测序和16 S rRNA基因定量PCR技术，对海南岛东部沿海上升流区（琼东上升流区）浮游细菌沿着的多样性、群落组成和16 S rRNA基因丰度进行了研究。与近海站相比，内陆架站具有更高的细菌基因丰度（高达3倍）和操作分类单元丰富度，这是上涌和较新鲜沃茨影响的结果。总体而言，大多数读数属于γ变形菌（11%-74%）和α变形菌（14%-43%）。盐度、溶解氧、NO3对细菌群落结构有显著影响，这也决定了涌升沃茨的理化特征。在水平方向上，富营养带中的杜鹃花科、SAR 86、蓝藻和拟杆菌的相对丰度和基因丰度由内向外陆架和陆坡逐渐降低，而交替单胞菌、弧菌和海洋藻科的相对丰度和基因丰度则相反。本文着重研究了河流对琼东上升流中浮游细菌多样性和分布的影响。
The diversity, community composition and 16S rRNA gene abundance of bacterioplankton along a transect across an upwelling area off the eastern coast of Hainan Island (the Qiongdong upwelling) were investigated in August of 2016 using high throughput sequencing and quantitative PCR assay of 16S rRNA genes. Compared with the offshore stations, the inner-shelf stations had higher bacterial gene abundance (up to 3 fold) and operational taxonomic unit richness, a result of the influence of upwelled and fresher waters. Overall, a majority of the reads were affiliated with Gammaproteobacteria (11%-74%) and Alphaproteobacteria (14%-43%). The structure of the bacterial community was significantly affected by salinity, dissolved oxygen, NO3, which also defined the physicochemical features of the upwelled waters. Horizontally, the relative abundances and gene abundances of Rhodobacteraceae, SAR86, Cyanobacteria and Bacteroidetes in eutrophic zone decreased from the inner to outer shelves and slope, whereas Alteromonas, Vibrio and Oceanospirillaceae exhibited an opposite trend. This study stresses the riverine influence on the oceanographic condition and spatial variability of bacterioplankton diversity and distribution in the Qiongdong upwelling.