CAGE (cap analysis of gene expression): a protocol for the detection of promoter and transcriptional networks.
CAGE (cap analysis of gene expression): a protocol for the detection of promoter and transcriptional networks.
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DOI:
10.1007/978-1-61779-292-2_11
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发表时间:
2012
期刊:
影响因子:
--
通讯作者:
Carninci, Piero
中科院分区:
文献类型:
--
作者:
Takahashi, Hazuki;Kato, Sachi;Murata, Mitsuyoshi;Carninci, Piero
We provide here a protocol for the preparation of cap-analysis gene expression (CAGE) libraries, which allow measuring the expression of eukaryotic capped RNAs and simultaneously map the promoter regions. The presented protocol simplified the previously published ones and moreover produces tags that are 27 nucleotides long, which facilitates mapping to the genome. The protocol takes less than 5 days to complete and presents a notable improvement compared to previously published versions.
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影响因子:
56.9
作者:
Carninci, P;Kasukawa, T;Hayashizaki, Y
通讯作者:
Hayashizaki, Y
影响因子:
30.8
作者:
Faulkner, Geoffrey J.;Kimura, Yasumasa;Carninci, Piero
通讯作者:
Carninci, Piero
影响因子:
2.7
作者:
Carninci, P;Shiraki, T;Hayashizaki, Y
通讯作者:
Hayashizaki, Y
影响因子:
4.4
作者:
Carninci, P;Kvam, C;Schneider, C
通讯作者:
Schneider, C
影响因子:
2.7
作者:
Shibata, Y;Carninci, P;Hayashizaki, Y
通讯作者:
Hayashizaki, Y