CAGE (cap analysis of gene expression): a protocol for the detection of promoter and transcriptional networks.

CAGE (cap analysis of gene expression): a protocol for the detection of promoter and transcriptional networks.
复制标题

DOI:
10.1007/978-1-61779-292-2_11
复制
发表时间:
2012
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Carninci, Piero
Carninci, Piero
中科院分区:
其他
文献类型:
--
作者:
Takahashi, Hazuki;Kato, Sachi;Murata, Mitsuyoshi;Carninci, Piero

文献摘要

参考文献

被引文献

相似文献

我们在这里提供了一种制备cap分析基因表达(CAGE)文库的方案,该文库允许测量真核生物的capped rna的表达,同时绘制启动子区域。所提出的方案简化了先前发表的方案,而且产生了27个核苷酸长的标签,这有利于定位到基因组。该协议只需不到5天的时间就可以完成,与以前发布的版本相比,它有了显着的改进。
We provide here a protocol for the preparation of cap-analysis gene expression (CAGE) libraries, which allow measuring the expression of eukaryotic capped RNAs and simultaneously map the promoter regions. The presented protocol simplified the previously published ones and moreover produces tags that are 27 nucleotides long, which facilitates mapping to the genome. The protocol takes less than 5 days to complete and presents a notable improvement compared to previously published versions.
DOI: 10.1126/science.1112014
发表时间: 2005-09-02
期刊: SCIENCE
影响因子: 56.9
作者:
Carninci, P;Kasukawa, T;Hayashizaki, Y
通讯作者: Hayashizaki, Y
DOI: 10.1038/ng.368
发表时间: 2009-05-01
期刊: NATURE GENETICS
影响因子: 30.8
作者:
Faulkner, Geoffrey J.;Kimura, Yasumasa;Carninci, Piero
通讯作者: Carninci, Piero
DOI: 10.2144/02325bm01
发表时间: 2002-05-01
期刊: BIOTECHNIQUES
影响因子: 2.7
作者:
Carninci, P;Shiraki, T;Hayashizaki, Y
通讯作者: Hayashizaki, Y
DOI: 10.1006/geno.1996.0567
发表时间: 1996-11-01
期刊: GENOMICS
影响因子: 4.4
作者:
Carninci, P;Kvam, C;Schneider, C
通讯作者: Schneider, C
DOI: 10.2144/01306st01
发表时间: 2001-06-01
期刊: BIOTECHNIQUES
影响因子: 2.7
作者:
Shibata, Y;Carninci, P;Hayashizaki, Y
通讯作者: Hayashizaki, Y