Role of the gibberellin receptors GID1 during fruit-set in Arabidopsis.
Role of the gibberellin receptors GID1 during fruit-set in Arabidopsis.
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DOI：
10.1111/tpj.12603
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发表时间：
2014-09
期刊：
影响因子：
--
通讯作者：
Perez-Amador MA
中科院分区：
文献类型：
--
作者：
Gallego-Giraldo C;Hu J;Urbez C;Gomez MD;Sun TP;Perez-Amador MA
赤霉素（GAs）在果实坐果和生长发育中起着重要作用。赤霉素被其核受体GA不敏感的DWARF 1 s（GID 1 s）感知，然后触发下游阻遏物DELLA的降解。为了了解拟南芥中3个GA受体基因（GID 1A、GID 1B和GID 1C）在果实发生过程中的作用，我们研究了它们的时空定位，并结合突变体表型分析。不同的表达模式，揭示了每个GID 1：GID 1A的整个雌蕊中表达，而GID 1B的胚珠中表达，和GID 1C的阀中表达。对gid 1突变体组合的功能研究证实，GID 1A在坐果和生长过程中起主要作用，而GID 1B和GID 1C分别在种子发育和荚果伸长中起特定作用。因此，在胚珠中，GA感知由GID 1A和GID 1B介导，而GID 1A和GID 1C参与瓣膜中的GA感知。为了确定GID 1和DELLA之间的组织特异性相互作用，我们分析了在果实起始中起作用的四个DELLA基因（GAI，RGA，RGL 1和RGL 2）的空间表达模式。我们的数据表明，GID 1A可以在所有组织中与RGA和GAI相互作用，而GID 1C-RGL 1和GID 1B-RGL 2相互作用仅分别发生在瓣膜和胚珠中。这些结果揭示了特定的功能，每个GID 1-DELLA在不同的GA依赖的过程中发生后坐果。此外，GA受体分布在阀沿着缺乏GA生物合成基因的表达，在这个组织中，强烈建议运输的GA从发展中的种子，以促进果实生长。
Gibberellins (GAs) play a critical role in fruit-set and fruit growth. Gibberellin is perceived by its nuclear receptors GA INSENSITIVE DWARF1s (GID1s), which then trigger degradation of downstream repressors DELLAs. To understand the role of the three GA receptor genes (GID1A, GID1B and GID1C) in Arabidopsis during fruit initiation, we have examined their temporal and spatial localization, in combination with analysis of mutant phenotypes. Distinct expression patterns are revealed for each GID1: GID1A is expressed throughout the whole pistil, while GID1B is expressed in ovules, and GID1C is expressed in valves. Functional study of gid1 mutant combinations confirms that GID1A plays a major role during fruit-set and growth, whereas GID1B and GID1C have specific roles in seed development and pod elongation, respectively. Therefore, in ovules, GA perception is mediated by GID1A and GID1B, while GID1A and GID1C are involved in GA perception in valves. To identify tissue-specific interactions between GID1s and DELLAs, we analyzed spatial expression patterns of four DELLA genes that have a role in fruit initiation (GAI, RGA, RGL1 and RGL2). Our data suggest that GID1A can interact with RGA and GAI in all tissues, whereas GID1C–RGL1 and GID1B–RGL2 interactions only occur in valves and ovules, respectively. These results uncover specific functions of each GID1–DELLA in the different GA-dependent processes that occur upon fruit-set. In addition, the distribution of GA receptors in valves along with lack of expression of GA biosynthesis genes in this tissue, strongly suggests transport of GAs from the developing seeds to promote fruit growth.
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