Hydrolyzed Salmon Milt Extract Enhances Object Recognition and Location Memory Through an Increase in Hippocampal Cytidine Nucleoside Levels in Normal Mice
Hydrolyzed Salmon Milt Extract Enhances Object Recognition and Location Memory Through an Increase in Hippocampal Cytidine Nucleoside Levels in Normal Mice
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DOI：
10.1089/jmf.2018.4285
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发表时间：
2019-04-01
影响因子：
2.4
通讯作者：
Kato, Yukio
中科院分区：
文献类型：
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作者：
Nakamichi, Noritaka;Nakao, Shunsuke;Kato, Yukio
鲑鱼鱼精提取物含有高水平的核酸，并具有抗氧化潜力。虽然鲑鱼鱼糜提取物已知可以改善脑疾病动物模型中受损的脑功能，但其对健康受试者学习和记忆能力的影响尚不清楚。本研究的目的是阐明水解鲑鱼鱼精提取物（HSME）的物体识别和物体位置记忆在正常条件下的影响。饮食含有2.5%的HSME诱导正常小鼠投入更多的时间来探索新的和移动的物体比探索熟悉的和不动的物体，观察期间的新物体识别和空间识别测试，分别。含有2.5%从HSME纯化的核酸组分的饮食也诱导了类似的效果，如通过相同的行为测试所测量的。这表明核酸可能是HSME对脑功能影响的功能组分。定量聚合酶链反应分析显示，在HSME饮食的小鼠脑实质细胞，包括神经干细胞，星形胶质细胞，少突胶质细胞和小胶质细胞的标志物的基因表达高于对照饮食的小鼠。口服HSME增加了海马中胞嘧啶、胞苷和脱氧胞苷的浓度。总体而言，摄取HSME可以增强小鼠在正常条件下的物体识别和物体位置记忆，至少部分是通过激活脑实质细胞。因此，我们的研究结果表明，饮食中摄入这种容易摄入的食物可能会增强健康个体的大脑功能。
Salmon milt extract contains high levels of nucleic acids and has antioxidant potential. Although salmon milt extract is known to improve impaired brain function in animal models with brain disease, its effects on learning and memory ability in healthy subjects is unknown. The purpose of the present study was to clarify the effect of hydrolyzed salmon milt extract (HSME) on object recognition and object location memory under normal conditions. A diet containing 2.5% HSME induced normal mice to devote more time to exploring novel and moved objects than in exploring familiar and unmoved objects, as observed during novel object recognition and spatial recognition tests, respectively. A diet containing 2.5% nucleic acid fraction purified from HSME also induced similar effects, as measured by the same behavioral tests. This suggests that the nucleic acids may be a functional component contributing to the effects of HSME on brain function. Quantitative polymerase chain reaction analysis revealed that gene expression of the markers for brain parenchymal cells, including neural stem cells, astrocytes, oligodendrocytes, and microglia, in the hippocampi of mice on an HSME diet was higher than that in mice on a control diet. Oral administration of HSME increased concentrations of cytosine, cytidine, and deoxycytidine in the hippocampus. Overall, ingestion of HSME may enhance object recognition and object location memory under normal conditions in mice, at least, in part, via the activation of brain parenchymal cells. Our results thus indicate that dietary intake of this easily ingestible food might enhance brain function in healthy individuals.