Species-specific wiring for direction selectivity in the mammalian retina.
Species-specific wiring for direction selectivity in the mammalian retina.
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DOI：
10.1038/nature18609
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发表时间：
2016-07-07
期刊：
影响因子：
64.8
通讯作者：
Briggman KL
中科院分区：
文献类型：
--
作者：
Ding H;Smith RG;Poleg-Polsky A;Diamond JS;Briggman KL
星爆无长突细胞（SAC）树突中的定向调谐信号位于哺乳动物视网膜中方向选择（DS）电路的核心。内在细胞特性和网络连接对 SAC DS 的相对贡献仍不清楚。我们提出了小鼠视网膜中 SAC 电路的详细连接组学重建，并描述了沿 SAC 树突的突触分布的先前未知特征：1）输入和输出突触是分离的，输入仅限于近端树突； 2）抑制输入的分布与在兔子视网膜中观察到的根本不同。解剖学约束的 SAC 网络模型表明，小鼠和兔子视网膜之间的 SAC-SAC 接线差异是突触抑制对速度和对比度调节以及感受野结构的不同贡献的基础。特别是，该模型表明，鼠标连接使 SAC 能够编码较低的线速度，从而减少眼睛直径，从而保留角速度调整。这些预测通过小鼠 SAC 树突响应定向刺激的钙成像得到证实。
Directionally tuned signaling in starburst amacrine cell (SAC) dendrites lies at the heart of the direction selective (DS) circuit in the mammalian retina. The relative contributions of intrinsic cellular properties and network connectivity to SAC DS remain unclear. We present a detailed connectomic reconstruction of SAC circuitry in mouse retina and describe previously unknown features of synapse distributions along SAC dendrites: 1) input and output synapses are segregated, with inputs restricted to proximal dendrites; 2) the distribution of inhibitory inputs is fundamentally different from that observed in rabbit retina. An anatomically constrained SAC network model suggests that SAC-SAC wiring differences between mouse and rabbit retina underlie distinct contributions of synaptic inhibition to velocity and contrast tuning and receptive field structure. In particular, the model indicates that mouse connectivity enables SACs to encode lower linear velocities that account for smaller eye diameter, thereby conserving angular velocity tuning. These predictions are confirmed with calcium imaging of mouse SAC dendrites in response to directional stimuli.