Identification of a novel collagen-like peptide by high-throughput screening for effective wound-healing therapy
Identification of a novel collagen-like peptide by high-throughput screening for effective wound-healing therapy
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通过高通量筛选鉴定新型胶原蛋白样肽，用于有效的伤口愈合治疗
DOI：
10.1016/j.ijbiomac.2021.01.104
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发表时间：
2021-01-27
影响因子：
8.2
通讯作者：
Qin, Yiru
中科院分区：
文献类型：
--
作者：
Du, Chunyu;Li, Ying;Qin, Yiru
组织再生和伤口愈合仍然是全球严重的临床并发症，缺乏令人满意的治疗方法。受到损伤后蚯蚓令人印象深刻的再生能力及其广泛接受的药用特性的启发，我们使用高通量技术，包括转录组学，蛋白质组学和质谱，从截肢蚯蚓中筛选并鉴定了一种新的胶原蛋白样肽。克隆并表达鉴定出的胶原样肽col 4a 1，以全面研究其对创伤愈合的作用及其机制。它在体外和体内均对伤口愈合产生显著影响，包括增强成纤维细胞的活力、增殖、迁移、肉芽形成和胶原沉积。此外，col 4a 1通过与整合素α（2）β（1）结合并上调RAS/MAPK信号通路发挥作用。这项工作表明，从截肢蚯蚓中获得的新型胶原蛋白样肽col 4a 1能够增强伤口愈合，并为伤口护理提供新的机会。(C)2021爱思唯尔有限公司版权所有。
Tissue regeneration and wound healing are still serious clinical complications globally and lack satisfactory cures. Inspired by the impressive regeneration ability of the post-injury earthworms and their widely accepted medicinal properties, we screened and identified a novel collagen-like peptide from the amputated earthworms using high-throughput techniques, including transcriptomics, proteomics, and mass spectrum. The identified collagen-like peptide col4a1 was cloned and expressed to comprehensively investigate the wound healing effect and underlying mechanism. It exerted significant effects on wound healing both in vitro and in vivo, including enhanced viability, proliferation, migration of fibroblasts, granulation, and collagen deposition. Moreover, the col4a1 functioned via binding with integrin alpha(2)beta(1) and upregulating the RAS/MAPK signaling pathway. This work demonstrates that the novel collagen-like peptide col4a1 obtained from the amputated earthworms enables enhanced wound healing and provides new opportunities for wound care. (C) 2021 Elsevier B.V. All rights reserved.