Size Matters: Ryanodine Receptor Cluster Size Affects Arrhythmogenic Sarcoplasmic Reticulum Calcium Release
Size Matters: Ryanodine Receptor Cluster Size Affects Arrhythmogenic Sarcoplasmic Reticulum Calcium Release
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DOI：
10.1161/jaha.118.008724
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发表时间：
2018-07-03
影响因子：
5.4
通讯作者：
Bers, Donald M.
中科院分区：
文献类型：
--
作者：
Galice, Samuel;Xie, Yuanfang;Bers, Donald M.
Ryanodine受体（RyR）介导肌浆网钙（Ca 2+）释放并影响肌细胞Ca 2+稳态和心律失常。在心肌细胞中，RyR以各种大小和形状的簇存在，RyR簇的大小可能严重影响正常和致心律失常的Ca-2+火花和波的形成。然而，在特定的钙火花网站的实际RyR簇的大小从来没有被测量在生理setting.Methods和结果-在这里，我们测量RyR簇的大小和钙火花同时评估RyR簇的大小如何影响钙火花和肌浆网Ca 2+泄漏。对于小RyR集群大小（
Background--Ryanodine receptors (RyR) mediate sarcoplasmic reticulum calcium (Ca2+) release and influence myocyte Ca2+ homeostasis and arrhythmias. In cardiac myocytes, RyRs are found in clusters of various sizes and shapes, and RyR cluster size may critically influence normal and arrhythmogenic Ca-2+ spark and wave formation. However, the actual RyR cluster sizes at specific Ca2+ spark sites have never been measured in the physiological setting.Methods and Results--Here we measured RyR cluster size and Ca2+ sparks simultaneously to assess how RyR cluster size influences Ca2+ sparks and sarcoplasmic reticulum Ca2+ leak. For small RyR cluster sizes (