SCREENING OF PGPR ISOLATES FROM SEMI-ARID REGION AND THEIR IMPLICATION TO ALLEVIATE DROUGHT STRESS
SCREENING OF PGPR ISOLATES FROM SEMI-ARID REGION AND THEIR IMPLICATION TO ALLEVIATE DROUGHT STRESS
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半干旱地区 PGPR 分离株的筛选及其对缓解干旱胁迫的意义
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Samiullah
中科院分区：
文献类型：
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作者：
H. Yasmin;A. Bano;Samiullah
本研究从巴基斯坦Kahuta地区土壤含水量为12%的半干旱区玉米根际土壤中分离出24株促进植物生长的根际细菌。这些菌株在菌落形态、革兰氏试验和过氧化氢酶试验的基础上差异很大。根据其产铁载体、p溶解和细菌素的阳性活性筛选最有效的细菌分离株。PGPR分离物9K溶磷指数最高。1K （1Kahuta）和KB （KahutaB）产生铁载体，而（KahutaB）也产生细菌素。选择PGPR分离株1k、KB和9K在诱导干旱胁迫条件下对玉米进行再接种研究。PGPR分离物9K通过促进根系增殖和提高叶片相对含水量提高了玉米植株的抗旱性，与接种对照相比，根冠干重比显著提高（26%）。PGPR分离物9K可用于干旱半干旱区生物肥料的配制，起到缓解干旱的作用。
During the present study isolation of 24 plant growth promoting rhizobacteria isolates were made from rhizosphere soil of maize collected from semi-arid region of Kahuta, Pakistan having 12% soil moisture. These isolates varied considerably on the basis of colony morphology, Gram’s test and catalase test. Most efficient bacterial isolates were screened on the basis of their positive activity for siderophore production, P-solubilization and bacteriocin production. The PGPR isolate 9K showed maximum P-solubilization index. Siderophore production was exhibited by 1K (1Kahuta) and KB (KahutaB) while (KahutaB) showed bacteriocin production also. The PGPR isolates1K, KB and 9K were selected for re-inoculation studies on maize under induced drought stress condition. The PGPR isolate 9K increased drought tolerance in maize plants by enhancing root proliferation and improving relative water content of leaves, significant (26%) increased in root to shoot dry weight ratio as compared to inoculated control. The PGPR isolate 9K can be selected in the formulation of bio-fertilizers for alleviating drought effects in arid and semi-arid region.