NEW APPROACH TO CALCULATION OF SECONDARY STRUCTURES OF GLOBULAR PROTEINS BY OPTICAL ROTATORY DISPERSION AND CIRCULAR DICHROISM
NEW APPROACH TO CALCULATION OF SECONDARY STRUCTURES OF GLOBULAR PROTEINS BY OPTICAL ROTATORY DISPERSION AND CIRCULAR DICHROISM
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DOI：
10.1016/s0006-291x(71)80225-5
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发表时间：
1971-01-01
影响因子：
3.1
通讯作者：
YANG, JT
中科院分区：
文献类型：
--
作者：
CHEN, YH;YANG, JT
旋转贡献可以写为X=Σfixi，其中下标i=H、β和R表示螺旋、β形式和无序形式的分数。X可以是莫菲特方程、[m]233、[θ]222或任何选定波长下的其他参数。利用X射线衍射研究中的FI，用最小二乘法求出参考值X。蛋白质的螺旋含量可由下列公式确定：(1)bo=−580fH，(2)[m]233=−12700fH−2520，和(3)[θ]222=−30300fH−2340。β形式的估计仍然是不确定的。
The rotatory contributions can be written as X = ΣfiXi, where the subscripts i = H, β and R represent the fractions of the helix, β-form and unordered form. X can be the boof the Moffitt equation, [m]233, [θ]222or other parameters at any chosen wavelength. With fi's from the X-ray diffraction studies, the reference values, X′s, are solved by a least-square method. The helical content of a protein can be determined by: (1) bo= −580fH, (2) [m]233= −12700fH−2520, and (3) [θ]222= −30300fH− 2340. The estimation of the β-form is still uncertain.