A laser-activated multifunctional targeted nanoagent for imaging and gene therapy in a mouse xenograft model with retinoblastoma Y79 cells
A laser-activated multifunctional targeted nanoagent for imaging and gene therapy in a mouse xenograft model with retinoblastoma Y79 cells
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一种激光激活的多功能靶向纳米制剂，用于视网膜母细胞瘤 Y79 细胞的小鼠异种移植模型中的成像和基因治疗
DOI：
10.1016/j.actbio.2018.02.006
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发表时间：
2018
影响因子：
9.7
通讯作者：
Xiyuan Zhou
中科院分区：
文献类型：
--
作者：
Mingxing Wu;Haibo Xiong;Hongmi Zou;Meng Li;Pan Li;Yu Zhou;Yan Xu;Jia Jian;Fengqiu Liu;Hongyun Zhao;Zhigang Wang;Xiyuan Zhou
视网膜母细胞瘤（Retinoblastoma， RB）是儿童最常见的眼内恶性肿瘤，迫切需要早期发现和有效治疗。纳米级基因传递系统的使用很有吸引力。由于其高度可调节的结构，可同时携带治疗和显像剂。在此,我们。报道了一种叶酸（FA）修饰的相变阳离子纳米颗粒包封液体全氟戊烷。（PFP）和吲哚菁绿（ICG） （FA-CN-PFP-ICG， FCNPI）具有良好的质粒DNA （pDNA）承载能力，良好的生物相容性，优异的光声（PA）和超声（US）对比。提高基因转染效率和治疗效果。FCNPI的液气相变。在激光照射下，为US/PA双模成像提供了良好的对比。体内。更重要的是，与非靶向阳离子纳米颗粒（CN-PFP-ICG,. cnpi）和中性纳米颗粒（NN-PFP-ICG， NNPI）相比，激光介导的靶向阳离子FCNPI纳米颗粒基因转染在体外和体内均表现出最佳的治疗效果。这样的多功能。纳米药物有望结合双模引导成像，副作用少，治疗效果好。这些结果为临床检测和治疗提供了实验依据。RB。
Retinoblastoma (RB) is the most common intraocular malignancy of childhood that urgently needs early.detection and effective therapy methods. The use of nanosized gene delivery systems is appealing.because of their highly adjustable structure to carry both therapeutic and imaging agents. Herein, we.report a folic acid (FA)-modified phase-changeable cationic nanoparticle encapsulating liquid perfluoropentane.(PFP) and indocyanine green (ICG) (FA-CN-PFP-ICG, FCNPI) with good plasmid DNA (pDNA).carrying capacity, favorable biocompatibility, excellent photoacoustic (PA) and ultrasound (US) contrast,.enhanced gene transfection efficiency and therapeutic effect. The liquid-gas phase transition of the FCNPI.upon laser irradiation has provided splendid contrasts for US/PA dual-modality imaging in vitro as well as.in vivo. More importantly, laser-mediated gene transfection with targeted cationic FCNPI nanoparticles.demonstrated the best therapeutic effect compared with untargeted cationic nanoparticle (CN-PFP-ICG,.CNPI) and neutral nanoparticle (NN-PFP-ICG, NNPI), both in vitro and in vivo. Such a multifunctional.nanoagent is expected to combine dual-mode guided imaging with fewer side effects and proper therapeutic.efficacy. These results establish an experimental foundation for the clinical detection of and therapy.for RB.