TRANSFORMING GROWTH-FACTOR-BETA - SELECTIVE INCREASE IN GLYCOSAMINOGLYCAN SYNTHESIS BY CULTURES OF FIBROBLASTS FROM PATIENTS WITH PROGRESSIVE SYSTEMIC-SCLEROSIS
TRANSFORMING GROWTH-FACTOR-BETA - SELECTIVE INCREASE IN GLYCOSAMINOGLYCAN SYNTHESIS BY CULTURES OF FIBROBLASTS FROM PATIENTS WITH PROGRESSIVE SYSTEMIC-SCLEROSIS
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DOI：
10.1111/1523-1747.ep12580445
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发表时间：
1987-07-01
影响因子：
6.5
通讯作者：
SPORN, MB
中科院分区：
文献类型：
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作者：
FALANGA, V;TIEGS, SL;SPORN, MB
到目前为止，在所有被检查的细胞中都发现了转化生长因子-β，并被证明在炎症和结缔组织的形成中起着重要的作用。我们现在报告，与正常成纤维细胞相比，Tcf-beta单独或与表皮生长因子联合使用能优先增加进行性系统性硬化症患者真皮成纤维细胞培养的糖胺多糖合成(p<；0.001)。转化生长因子-β对PSS和正常成纤维细胞的胶原合成均有相同程度的促进作用，而EGF对胶原合成无刺激作用。与单独使用转化生长因子β相比，在与转化生长因子-β共同孵育的培养物中加入表皮生长因子导致胶原合成减少(P<；0.02)。这些研究表明，转化生长因子-β可能在PSS结缔组织的聚集中起重要作用，多种生长因子的联合作用可能调节人真皮成纤维细胞的合成活性。
Transforming growth factor-beta (TGF-beta) has been found in all cells examined thus far, and has been shown to play an important role in inflammation and connective tissue formation. We now report TCF-beta, alone or in combination with epidermal growth Factor (EGF), led to a preferential increase in glycosaminoglycan synthesis by cultures of dermal fibroblasts from patients with progressive systemic sclerosis (PSS) when compared with normal fibroblasts (p < 0.001). Tranforming growth factor-beta increased collagen synthesis to the same extent in both PSS and normal fibroblasts, whereas EGF had no stimulatory activity on collagen synthesis. The addition of EGF to cultures incubated with TGF-beta led to a decrease in collagen synthesis compared with the effect seen with TGF-beta alone (P < 0.02). These studies suggest that TGF-beta may play an important role in the accumulation of connective tissue seen in PSS and that the combined action of multiple growth factors may modulate the synthetic activity of human dermal fibroblasts.