Intratumoral Injection of BCG-CWS-Pretreated Dendritic Cells Following Tumor Cryoablation
Intratumoral Injection of BCG-CWS-Pretreated Dendritic Cells Following Tumor Cryoablation
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肿瘤冷冻消融后瘤内注射 BCG-CWS 预处理的树突状细胞
DOI：
10.1007/978-1-4939-0345-0_13
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Kawakami Y.
中科院分区：
文献类型：
--
作者：
Kawamura N;Udagawa M;Fujita T;Sakurai T;Yaguchi T;Kawakami Y.
肿瘤冷冻消融后瘤内注射树突状细胞（DC）是一种个性化的肿瘤免疫治疗方法，能够诱导针对多种内源性肿瘤抗原（包括共享和独特抗原）的免疫应答。在这里，我们描述的协议冷冻消融肿瘤，产生培养的DC，预处理DC与Toll样受体（TLR）刺激纯化的成分卡介苗细胞壁部分（BCG-CWS）和高免疫原性匙孔血蓝蛋白（KLH）抗原，并结合使用肿瘤冷冻消融和瘤内给药BCG-CWS预处理的DC在小鼠模型和癌症患者。
Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.