Spectral behavior of second harmonic signals from organic and non-organic materials in multiphoton microscopy
Spectral behavior of second harmonic signals from organic and non-organic materials in multiphoton microscopy
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DOI：
10.1063/1.4915134
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发表时间：
2015-08-01
期刊：
影响因子：
1.6
通讯作者：
Heisterkamp, Alexander
中科院分区：
文献类型：
--
作者：
Ehmke, Tobias;Knebl, Andreas;Heisterkamp, Alexander
由于非线性信号的光谱分离，多模态非线性显微镜允许对高度有序的生物组织进行成像。这需要关于不同非线性信号的频谱分布的某些知识。在几个出版物相比，我们证明了一个因素的1/2根2有关的高斯激光脉冲光谱的半高全宽相应的二次谐波脉冲光谱在空间域中通过使用一个简单的理论模型。磷酸二氢钾晶体（KDP晶体）和猪角膜组织的实验支持我们的理论预测。此外，没有发现二次谐波信号的epi-和trans-detection的谱宽度的差异。总体而言，这些结果可能有助于建立一个优化的多光子设置的非线性信号的光谱分离。(C)2015年作者。所有文章内容，除非另有说明，是根据知识共享署名3.0未移植许可证许可。
Multimodal nonlinear microscopy allows imaging of highly ordered biological tissue due to spectral separation of nonlinear signals. This requires certain knowledge about the spectral distribution of the different nonlinear signals. In contrast to several publications we demonstrate a factor of 1/2 root 2 relating the full width at half maximum of a gaussian laser pulse spectrum to the corresponding second harmonic pulse spectrum in the spatial domain by using a simple theoretical model. Experiments on monopotassium phosphate crystals (KDP-crystals) and on porcine corneal tissue support our theoretical predictions. Furthermore, no differences in spectral width were found for epi- and trans-detection of the second harmonic signal. Overall, these results may help to build an optimized multiphoton setup for spectral separation of nonlinear signals. (C) 2015 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.