GlbN (cyanoglobin) is a peripheral membrane protein that is restricted to certain Nostoc spp
GlbN (cyanoglobin) is a peripheral membrane protein that is restricted to certain Nostoc spp
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GlbN（氰红蛋白）是一种仅限于某些发菜属的外周膜蛋白
作者：
D. R. Hill;T. Belbin;M. Thorsteinsson;Deeni Bassam;S. Brass;A. Ernst;P. Boger;H. Paerl;M. E. Mulligan;M. Potts
发菜UTEX 584的glbN基因与nifU和nifH并列，编码一个12 kDa的单体血红蛋白，与氧有很高的亲和力。在N.Comme UTEX 584中，当放氧速率被抑制到低于25微摩尔O2 mg的叶绿素a(-1)h(-1)时，固氮培养的异形胞和营养细胞都出现了GlbN的最大积累。GlbN的积累与nifH和铁还蛋白NADP+氧化还原酶(peth或fnr)的最大合成量相吻合。共有41株蓝藻进行了GlbN或GlbN的筛选，其中包括40株固氮菌，代表了所有五个蓝藻的16个属。GlbN在5个发菜菌株中以单拷贝形式存在。11株发菜和鱼腥藻的基因组DNA，包括鱼腥藻。PCC7120菌株不提供与glbN探针的杂交信号。在4个鱼腥藻和发菜菌株以及塞氏毛霉中检测到一种成分表达的18 kDa蛋白，该蛋白与GlbN抗体发生强烈的交叉反应。发菜nifU-nifH基因间隔区对MUN 8820株进行了序列测定(1,229个碱基)，与N.Comme UTEX 584中的相应区域大约有95%的同源性。两个菌株nifU-nifH基因间隔区的每条DNA链都有可能折叠成二级结构，其中50%以上的碱基是内部配对的。迁移率移动分析证实，NTCA(BIFA)结合在公社UTEX 584的nifU-glbN基因间隔区，位于glbN翻译起始点上游约100个碱基。该序列与聚球藻的glnA启动子序列具有广泛的相似性。PCC7942菌株。在体内，GlbN在细胞膜的胞浆面周围有一个特殊而显著的亚细胞位置，该蛋白仅存在于细胞提取物的可溶部分中。我们的假设是，GlbN清除氧气，是膜相关的微需氧诱导的末端细胞色素氧化酶的组成部分。
The glbN gene of Nostoc commune UTEX 584 is juxtaposed to nifU and nifH, and it encodes a 12-kDa monomeric hemoglobin that binds oxygen with high affinity. In N. commune UTEX 584, maximum accumulation of GlbN occurred in both the heterocysts and vegetative cells of nitrogen-fixing cultures when the rate of oxygen evolution was repressed to less than 25 micromol of O2 mg of chlorophyll a(-1) h(-1). Accumulation of GlbN coincided with maximum synthesis of NifH and ferredoxin NADP+ oxidoreductase (PetH or FNR). A total of 41 strains of cyanobacteria, including 40 nitrogen fixers and representing 16 genera within all five sections of the cyanobacteria were screened for the presence of glbN or GlbN. glbN was present in five Nostoc strains in a single copy. Genomic DNAs from 11 other Nostoc and Anabaena strains, including Anabaena sp. strain PCC 7120, provided no hybridization signals with a glbN probe. A constitutively expressed, 18-kDa protein which cross-reacted strongly with GlbN antibodies was detected in four Anabaena and Nostoc strains and in Trichodesmium thiebautii. The nifU-nifH intergenic region of Nostoc sp. strain MUN 8820 was sequenced (1,229 bp) and was approximately 95% identical to the equivalent region in N. commune UTEX 584. Each strand of the DNA from the nifU-nifH intergenic regions of both strains has the potential to fold into secondary structures in which more than 50% of the bases are internally paired. Mobility shift assays confirmed that NtcA (BifA) bound a site in the nifU-glbN intergenic region of N. commune UTEX 584 approximately 100 bases upstream from the translation initiation site of glbN. This site showed extensive sequence similarity with the promoter region of glnA from Synechococcus sp. strain PCC 7942. In vivo, GlbN had a specific and prominent subcellular location around the periphery of the cytosolic face of the cell membrane, and the protein was found solely in the soluble fraction of cell extracts. Our hypothesis is that GlbN scavenges oxygen for and is a component of a membrane-associated microaerobically induced terminal cytochrome oxidase.
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DOI：
10.1016/s0021-9258(18)61070-1
发表时间：
1987-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Matsudaira
通讯作者：
P. Matsudaira
原核生物中发育调控的基因重排。
影响因子：
11.1
作者：
Haselkorn,R
通讯作者：
Haselkorn,R
DOI：
10.1016/s0021-9258(19)47287-6
发表时间：
1989-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. E. Mulligan;Robert Haselkorn
通讯作者：
M. E. Mulligan;Robert Haselkorn
DOI：
10.1073/pnas.86.20.7706
发表时间：
1989-10-01
影响因子：
11.1
作者：
JAEGER, JA;TURNER, DH;ZUKER, M
通讯作者：
ZUKER, M
预测 RNA 的最佳和次优二级结构。
影响因子：
--
作者：
Jaeger,JA;Turner,DH;Zuker,M
通讯作者：
Zuker,M