Pax3 and Pax7 interact reciprocally and regulate the expression of cadherin-7 through inducing neuron differentiation in the developing chicken spinal cord
Pax3 and Pax7 interact reciprocally and regulate the expression of cadherin-7 through inducing neuron differentiation in the developing chicken spinal cord
复制标题
DOI：
10.1002/cne.23885
复制
发表时间：
2016-04-01
影响因子：
2.5
通讯作者：
Redies, Christoph
中科院分区：
文献类型：
--
作者：
Lin, Juntang;Wang, Congrui;Redies, Christoph
Pax 3和Pax 7是两个密切相关的转录因子，在发育中的神经系统和体节中广泛表达。在中枢神经系统中，这两种基因在发育过程中在神经管的背侧部分表达。Pax 3和Pax 7参与音刺猬（Shh）信号通路，并被Shh过表达抑制。本研究证实，在体内，Pax 3过表达抑制Pax 7的表达，而Pax 7过表达内源性增强和异位诱导发育中的鸡脊髓中的Pax 3的表达。Pax 3和Pax 7的过表达抑制了钙粘蛋白-7（形态发生基因的钙粘蛋白家族的成员）的内源性表达，并诱导其异位表达。本研究还表明，Pax 3和Pax 7的过度表达改变了神经上皮层细胞的命运和形态，并诱导有丝分裂后神经元标记物的表达。我们发现Pax 3和Pax 7都能促进神经前体细胞分化为神经元。此外，Pax 3和Pax 7与特定的shRNA下调导致发育中的脊髓细胞凋亡。总的来说，这些结果表明，转录因子Pax 3和Pax 7在调节发育中的脊髓的形态发生和细胞分化中发挥重要作用。神经学比较杂志524：940-962，2016. (c)2015 Wiley Periodicals，Inc.
Pax3 and Pax7 are closely related transcription factors that are widely expressed in the developing nervous system and somites. In the CNS, both genes are expressed in the dorsal part of the neural tube during development. Pax3 and Pax7 are involved in the sonic hedgehog (Shh) signaling pathway and are inhibited by Shh overexpression. The present study confirms in vivo that Pax3 overexpression represses the expression of Pax7, whereas Pax7 overexpression endogenously enhances and ectopically induces the expression of Pax3 in the developing chicken spinal cord. Overexpression of Pax3 and Pax7 represses the endogenous expression of cadherin-7, a member of the cadherin family of morphogenetic genes, and induces its ectopic expression. The present study also shows that overexpression of Pax3 and Pax7 changes the fate and morphology of cells in the neuroepithelial layer and induces the expression of postmitotic neuronal markers. We show that both Pax3 and Pax7 promote the differentiation of neural progenitor cells into neurons. Furthermore, the downregulation of Pax3 and Pax7 with specific shRNAs results in apoptosis in the developing spinal cord. Collectively, these results suggest that the transcription factors Pax3 and Pax7 play important roles in regulating morphogenesis and cell differentiation in the developing spinal cord. J. Comp. Neurol. 524:940-962, 2016. (c) 2015 Wiley Periodicals, Inc.