Therapeutic perspectives of in vivo cell electropermeabilization
Therapeutic perspectives of in vivo cell electropermeabilization
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DOI：
10.1016/s0302-4598(00)00112-4
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发表时间：
2001-01-01
影响因子：
5
通讯作者：
Mir, LM
中科院分区：
文献类型：
--
作者：
Mir, LM
细胞电透化（也称为细胞电穿孔）是目前用于生物化学和药理学研究的常规技术，用于将非渗透分子体外引入活细胞中。但是电脉冲也可以在体内用于将药物或DNA递送到组织细胞中。然后，本文综述了体内细胞电透化的治疗前景，特别是抗肿瘤电化学疗法（即，细胞毒性非渗透性药物与递送至肿瘤的渗透性电脉冲的组合）和用于基因治疗的体内DNA电转移。在对细胞电透化（特别是在体内）的现有知识进行简短总结后，描述和讨论了电化学疗法的基础、目前的成就和挑战，其中包括对仍然悬而未决的问题的概述和对最近临床试验的更新。用于基因治疗的DNA电转移是一个新兴的领域，其结果正在迅速积累。目前的知识，DNA电转移机制，以及潜在的DNA电转移成为一个有效的非病毒的基因治疗方法，进行了审查。(C)2000 Elsevier Science S.A. All rights reserved.
Cell electropermeabilization (also termed cell electroporation) is nowadays a routine technique used in biochemical and pharmacological studies for the in vitro introduction of nonpermeant molecules into living cells. But electric pulses can be used as well in vivo for the delivery of drugs or DNA into cells of tissues. This review then gives an updated overview of the therapeutic perspectives of cell electropermeabilization in vivo, in particular of the antitumour electrochemotherapy (i.e., the combination of a cytotoxic nonpermeant drug with permeabilizing electric pulses delivered to the tumours) and of in vivo DNA electrotransfer for gene therapy. After a short summary of the present knowledge on cell electropermeabilization (particularly in vivo), the basis, the present achievements, and the challenges of electrochemotherapy are described and discussed, which includes an overview of still open questions and an update on recent clinical trials. DNA electrotransfer for gene therapy is an emerging field in which results are rapidly accumulating. Present knowledge on DNA electrotransfer mechanisms, as wel as the potentialities of DNA electrotransfer to become an efficient non-viral approach for gene therapy, are reviewed. (C) 2000 Elsevier Science S.A. All rights reserved.