Preparation of highly porous hydroxyapatite from cuttlefish bone
Preparation of highly porous hydroxyapatite from cuttlefish bone
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DOI：
10.1007/s10856-008-3674-0
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发表时间：
2009-05-01
影响因子：
3.7
通讯作者：
Ivankovic, M.
中科院分区：
文献类型：
--
作者：
Ivankovic, H.;Gallego Ferrer, G.;Ivankovic, M.
在200℃的高温下，以墨鱼骨的形式对文石进行水热处理，制备出用于组织工程的羟基磷灰石结构。经过48小时的水热处理，文石（CaCO3）单体石完全转化为羟基磷灰石。FT-IR光谱和Rietveld结构精化表明，Ca-10(PO4)(6)(OH)(2)结构的PO4(3)位点主要被CO3（2-）基团取代。随着高温处理时间的延长，nu 3PO4（3-）谱带强度增大，而nu 2CO3（2-）谱带强度减小，形成含羟基磷灰石的碳酸盐。扫描电镜观察结果表明，转换后的墨鱼骨中仍保持着由柱连接平行片层的相互连接的中空结构。高温处理增加了比表面积（S (BET)）和总孔容，降低了平均孔径。
Hydroxyapatite structures for tissue engineering applications have been produced by hydrothermal (HT) treatment of aragonite in the form of cuttlefish bone at 200A degrees C. Aragonite (CaCO3) monoliths were completely transformed into hydroxyapatite after 48 h of HT treatment. The substitution of CO3 (2-) groups predominantly into the PO4 (3-) sites of the Ca-10(PO4)(6)(OH)(2) structure was suggested by FT-IR spectroscopy and Rietveld structure refinement. The intensity of the nu 3PO4 (3-) bands increase, while the intensity of the nu 2CO3 (2-) bands decrease with the duration of HT treatment resulting in the formation of carbonate incorporating hydroxyapatite. The SEM micrographs have shown that the interconnected hollow structure with pillars connecting parallel lamellae in cuttlefish bone is maintained after conversion. Specific surface area (S (BET)) and total pore volume increased and mean pore size decreased by HT treatment.