Evidence for hexasomic inheritance in Chrysanthemum morifolium Ramat. based on analysis of EST-SSR markers
Evidence for hexasomic inheritance in Chrysanthemum morifolium Ramat. based on analysis of EST-SSR markers
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DOI：
10.1139/gen-2020-0155
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发表时间：
2022-02-01
期刊：
影响因子：
3.1
通讯作者：
Zhang, Qixiang
中科院分区：
文献类型：
--
作者：
Fan, Min;Gao, Yike;Zhang, Qixiang
菊花（Chrysanthemum morifolium Ramat.）是世界著名的观赏花卉。遗传方式对多倍体物种的遗传分析具有重要意义。然而，菊花的遗传分析一直受到阻碍，因为其有争议的遗传方式（二体或六体）。利用223个EST-SSR标记对192个小麦杂种F1代进行了3种方法的遗传模式分析。分析了EST-SSR标记等位基因的分离分析、单标记等位基因的偶联率和排斥率以及EST-SSR标记等位基因的传递和分离模式。经分离分析，204个标记等位基因符合六体遗传，150个标记等位基因符合二体遗传，表明标记等位基因主要以六体方式遗传。此外，对5个EST-SSR标记的等位基因构型和分离行为的分析结果也表明了染色体的随机配对。此外，单标记等位基因的比例为1：0，进一步支持六体遗传。因此，可以推断菊花是一个完整或接近完整的六分体。
Chrysanthemums (Chrysanthemum morifolium Ramat.) are ornamental flowers, which are famous worldwide. The mode of inheritance has great implications for the genetic analysis of polyploid species. However, genetic analysis of chrysanthemum has been hampered because of its controversial inheritance mode (disomic or hexasomic). To classify the inheritance mode of chrysanthemums, an analysis of three approaches was carried out in an F1 progeny of 192 offspring using 223 expressed sequence tag-simple sequence repeat (EST-SSR) markers. The analysis included segregation analysis, the ratio of simplex marker alleles linked in coupling to repulsion, as well as the transmission and segregation patterns of EST-SSR marker alleles. After segregation analysis, 204 marker alleles fit hexasomic inheritance and 150 marker alleles fit disomic inheritance, showing that marker alleles were inherited predominantly in a hexasomic manner. Furthermore, the results of the analysis of allele configuration and segregation behavior of five EST-SSR markers also suggested random pairing of chromosomes. Additionally, the ratio of simplex marker alleles linked in coupling to repulsion was 1:0, further supporting hexasomic inheritance. Therefore, it could be inferred that chrysanthemum is a complete or near-complete hexasome.