Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells.
Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells.
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中国仓鼠卵巢细胞紫外线敏感DNA修复突变体的遗传多样性。
DOI：
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发表时间：
1981
影响因子：
11.1
通讯作者：
Donald A. Glaser
中科院分区：
文献类型：
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作者：
L. H. Thompson;D. B. Busch;K. Brookman;Carolyn L. Mooney;Donald A. Glaser
用遗传互补分析方法对对紫外光杀灭和诱变敏感的中国仓鼠卵巢细胞突变系进行分析，以确定不同基因座的缺陷是否可能是共同的细胞表型的基础。为了便于快速筛选突变克隆，设计了一种程序，允许根据聚乙二醇融合后抗紫外线细胞的频率来评估假定的互补。在测试的44个无性系中，鉴定出4个类别。通过使用抗药性标记对UV突变体与野生型杂交的杂交细胞进行选择，这四个类别中的每个突变体都表现为表型隐性。还从四个突变体的每对组合之间的杂交中分离出杂交种。所有这些杂交种都对紫外线杀灭具有相对的抗性，这证实了互补类。当代表四个UV互补类别的突变体与多芳烃7-溴甲基苯并(A)菲进行测试时，所有配对组合都再次看到互补。这些结果表明，每一类突变体都代表一种生化缺陷，在修复紫外线诱导和化学诱导的DNA损伤方面发挥着共同的作用。
Mutant lines of Chinese hamster ovary cells that show hypersensitivity to killing and mutagenesis by UV light were analyzed by genetic complementation analysis to determine whether defects in different gene loci might underlie a common cellular phenotype,. To facilitate rapid screening of mutant clones, a procedure was devised that allowed presumptive complementation to be assessed on the basis of the frequency of UV-resistant cells after fusion by polyethylene glycol. Four classes were identified among 44 clones tested. By using drug-resistance markers for selection of hybrid cells in crosses between UV mutant and wild type, a mutant from each of the four classes was shown to behave as phenotypically recessive. Hybrids were also isolated from crosses between each of the pair combinations of the four mutants. All such hybrids were relatively resistant to UV killing, providing confirmation of the complementation classes. When mutants representing the four UV-complementation classes were tested with the polyaromatic hydrocarbon 7-bromomethylbenz(a)anthracene, complementation was again seen for all pair combinations. These results suggest that each class of mutants represents a biochemical defect that plays a common role in the repair of both UV-induced and chemically induced lesions in the DNA.