Demonstration of CDX2 is Highly Antibody Dependant
Demonstration of CDX2 is Highly Antibody Dependant
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DOI：
10.1097/pai.0b013e318257f8aa
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发表时间：
2013-01-01
影响因子：
1.6
通讯作者：
Vyberg, Mogens
中科院分区：
文献类型：
--
作者：
Borrisholt, Martine;Nielsen, Soren;Vyberg, Mogens
目的：CDX2 是一种广泛用于肿瘤肠道分化的免疫组织化学标记物。在 Nordic 免疫组织化学质量控制外部质量评估方案中，参与 2009 年 CDX2 挑战的实验室中只有 45% 产生了足够的染色。染色结果不足的主要原因似乎是一抗 (Ab) 克隆不太成功。为了以标准化方式评估抗体性能，进行了比较研究。材料和方法：使用包含309个非肿瘤组织和肿瘤样本的组织微阵列，其具有预期的高、低和无CDX2表达。选择五种抗体进行比较：EPR2764Y 浓缩 (Conc)、即用型形式的 EPR2764Y 以及 DAK-CDX2、AMT28 和 CDX2-88（均为浓缩）。使用 H 评分方法对 CDX2 染色进行盲评分。最大H分数为150至300（基于给予最高分数的染色）的组织/肿瘤样本被分类为CDX2高表达者，最大H分数为10至149的样本为低表达者，以及最大H分数的样本
Objects: CDX2 is a widely used immunohistochemical marker for intestinal differentiation in neoplasms. In the Nordic Immunohistochemical Quality Control external quality assessment scheme, only 45% of the laboratories participating in the CDX2 challenge in 2009 produced sufficient staining. A major cause of insufficient staining results appeared to be less successful primary antibody (Ab) clones. To evaluate the Ab performance in a standardized way, a comparative study was carried out.Materials and Methods: Tissue microarrays containing 309 nonneoplastic tissues and tumor samples with expected high, low, and no CDX2 expression were used. Five Abs were selected for comparison: EPR2764Y concentrated (Conc), EPR2764Y in a ready-to-use format, and DAK-CDX2, AMT28, and CDX2-88, all Conc. The CDX2 stains were scored blindly using the H-score method. Tissue/tumor samples with a maximum H-score of 150 to 300 (on the basis of the staining giving the highest score) were classified as CDX2 high expressors, samples with a maximum H-score of 10 to 149 as low expressors, and samples with a maximum H-score