Early onset of a decreased intracellular magnesium and phosphate concentration in smooth muscle cell of SHR.
Early onset of a decreased intracellular magnesium and phosphate concentration in smooth muscle cell of SHR.
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发表时间：
2005-12
影响因子：
3.2
通讯作者：
K. Kisters;F. Wessels;E. Krefting;B. Gremmler;M. Q. Nguyen;J. Kozianka;P. Franitza;F. Tokmak;M. Hausberg
中科院分区：
文献类型：
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作者：
K. Kisters;F. Wessels;E. Krefting;B. Gremmler;M. Q. Nguyen;J. Kozianka;P. Franitza;F. Tokmak;M. Hausberg
总镁含量的降低并不是镁离子浓度降低的直接证明。这可能反映了磷酸盐的改变或ATP代谢紊乱。自发性高血压大鼠（SHRs）的血浆磷酸盐水平低于Wistar-Kyoto大鼠（WKYs），并出现膜调节或线粒体ATP合酶缺陷。关于高血压细胞中镁和磷酸盐浓度的数据很少。采用电子探针x射线显微分析（Camscan CS 24仪器，剑桥，英国）测定10只m<s:1> nster鼠和10只年龄匹配的正常血压的WKY大鼠的主动脉平滑肌细胞内的磷酸盐和镁含量。SHRs的Mg++含量为0.09 +/- 0.15 g/kg干重，WKY的Mg++含量为1.15 +/- 0.10 g/kg干重（p < 0.01）。WKY大鼠血管平滑肌磷酸盐含量为23.6 +/- 0.79 g/kg干重，SHRs大鼠为15.81 +/- 1.22 g/kg干重（p < 0.01）。1月龄SHRs主动脉平滑肌细胞中，细胞内镁含量分别为1.05 +/- 0.08 g/kg和1.09 +/- 0.09 g/kg干重。1月龄SHRs的细胞内磷酸盐浓度为18.71 +/- 2.41，而WKYs的细胞内磷酸盐浓度为21.36 +/- 1.25 g/kg干重（每组8只）。SHRs的主动脉平滑肌细胞的特征是细胞内磷酸盐和镁浓度显著降低，导致atp代谢改变，如前所述。在原发性高血压的发病机制中，膜缺陷、镁缺乏或镁通道紊乱可能是早期发病的原因。
A decrease in total magnesium content is not a direct proof of a decreased magnesium ion concentration. It could reflect a phosphate alteration or an ATP metabolism disorder. Plasma phosphate levels are lower in spontaneously hypertensive rats (SHRs) than in Wistar-Kyoto rats (WKYs), and defects in membrane regulation or mitochondrial ATP synthase occur. Only sparse data exist concerning cellular magnesium and phosphate concentrations in hypertensive cells. In aortic smooth muscle cells from 10 SHRs of the Münster strain and 10 age-matched normotensive WKY rats, the intracellular phosphate and magnesium content was measured by electron probe X-ray microanalysis (Camscan CS 24 apparatus, Cambridge, U.K.). The Mg++ content was 0.09 +/- 0.15 g/kg dry weight in SHRs versus 1.15 +/- 0.10 g/kg dry weight in WKY rats (p < 0.01). Vascular smooth muscle phosphate content was 23.6 +/- 0.79 g/kg dry weight in WKY rats versus 15.81 +/- 1.22 g/kg dry weight in SHRs (p < 0.01). In aortic smooth muscle cells of one month old SHRs intracellular magnesium was measured as 1.05 +/- 0.08 versus 1.09 +/- 0.09 g/kg dry weight in WKYs. Intracellular phosphate concentration in one month old SHRs was 18.71 +/- 2.41 versus 21.36 +/- 1.25 g/kg dry weight in WKYs (eight animals in each group). Aortic smooth muscle cells of SHRs are caracterized by markedly lowered intracellular phosphate and magnesium concentrations, resulting in an altered ATP-metabolism, as described earlier. Possibly a membrane defect or a magnesium deficiency or disturbed magnesium channels are responsible for the early onset in the pathogenesis of primary hypertension.