Inhibition of rabies virus infection by an oligodeoxynucleotide complementary to rabies virus genomic RNA.
Inhibition of rabies virus infection by an oligodeoxynucleotide complementary to rabies virus genomic RNA.
复制标题
通过与狂犬病病毒基因组RNA互补的寡脱氧核苷酸抑制狂犬病病毒感染。
DOI：
10.1089/oli.1.1996.6.87
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Koprowski,H
中科院分区：
文献类型：
--
作者：
Fu,ZF;Wickstrom,E;Jiang,M;Corisdeo,S;Yang,J;Dietzschold,B;Koprowski,H
To develop antirabies virus-specific agents, eight oligodeoxynucleotides (ODN) complementary to either rabies virus genomic RNA (negative polarity) or rabies virus transcripts (mRNA) were synthesized and tested for their activity to inhibit rabies virus infection in cell cultures. It was found that the ODN RH+1 complementary to rabies virus genomic RNA blocked almost completely rabies virus infection at concentrations as low as 2 μM, whereas ODN complementary to viral transcripts did poorly even at concentrations as high as 20 μM. The antigenomic ODN also has the ability to inhibit cell-to-cell spread of rabies virus, which is an indicator for protection of rabies virus infectionin vivo. These results indicate that ODN complementary to rabies virus genomic RNA have strong ability to inhibit rabies virus infection in cell culture and may have the potential to be used for therapy in clinical rabies.
