SECRETION OF A NEW BASAL LAYER OF TECTORIAL MEMBRANE FOLLOWING GENTAMICIN-INDUCED HAIR CELL LOSS
SECRETION OF A NEW BASAL LAYER OF TECTORIAL MEMBRANE FOLLOWING GENTAMICIN-INDUCED HAIR CELL LOSS
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DOI：
10.1016/0378-5955(95)00141-9
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发表时间：
1995-10-01
期刊：
影响因子：
2.8
通讯作者：
COTANCHE, DA
中科院分区：
文献类型：
--
作者：
EPSTEIN, JE;COTANCHE, DA
采用扫描电镜（SEM）和视频增强DIC光镜观察庆大霉素诱导毛细胞脱落后鸡被膜（TM）的形态学变化。给药庆大霉素（100 mg/kg/天，连续3天），在初始注射后第5天和第10天检测固定和非固定制剂中分离的和原位的TMs。虽然该方案诱导毛细胞损伤延伸至基底乳头长度的75%，但对基底乳头本身没有明显的损伤。然而，受损毛细胞的排出似乎切断了基底乳头与基底乳头顶端表面之间的丝状附着。在SEM制备中，这种脱离导致TM向上边缘收缩。有趣的是，尽管没有TM损伤，庆大霉素治疗确实显示了TM新基底层的分泌。这个新的基底层在第5天开始分泌，到第10天组织良好。这一新层与恢复的基底乳头和覆盖的原始TM形成附着，这一步骤被认为是恢复再生耳蜗听觉功能所必需的。
Scanning electron microscopy (SEM) and video-enhanced DIC light microscopy were used to assess morphological changes in the chick tectorial membrane (TM) following gentamicin-induced hair cell loss. Gentamicin was administered (100 mg/kg/day for 3 days) and isolated and in-situ TMs were examined in both fixed and unfixed preparations at days 5 and 10 after the initial injection. Although this protocol induced hair cell damage extending up to 75% of the length of the basilar papilla, there was no apparent damage to the TM itself. However, the ejection of damaged hair cells appeared to sever the filamentous attachments between the TM and the apical surface of the basilar papilla. In SEM preparations this detachment caused the TM to shrink back toward the superior edge. Interestingly, despite the lack of TM damage, gentamicin treatment did reveal the secretion of a new basal layer of TM. Secretion of this new basal layer had begun by day 5 and it was well organized by day 10. This new layer formed attachments to both the recovering basilar papilla and the overlying original TM, a step thought to be necessary for the restoration of auditory function in the regenerating cochlea.