Identification and characterization of a glossy mutant in Welsh onion (Allium fistulosum L.)
Identification and characterization of a glossy mutant in Welsh onion (Allium fistulosum L.)
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DOI：
10.1016/j.scienta.2017.05.014
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发表时间：
2017-11
影响因子：
4.3
通讯作者：
Liuyi Yang;Qianchun Liu;Yongqin Wang;Lecheng Liu
中科院分区：
文献类型：
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作者：
Liuyi Yang;Qianchun Liu;Yongqin Wang;Lecheng Liu
覆盖在陆生植物初生气生器官上的角质层蜡由表皮蜡和表皮内蜡组成;这种蜡保护植物并影响许多生理特性。葱花是一种有光泽、鲜绿绿色的扁干葱。（GLBG）是由田间自然变异产生的，初步证明GLBG或光泽表型是由一对隐性基因控制的。对与角质层蜡质有关的生理特性进行了分析，结果表明，GLBG与糯质边干大葱（BG）在叶表面蜡质晶体的密度和形状上存在明显差异，其上表皮蜡质覆盖率比BG低50%。但在角质层膜透性和气孔密度上差异不显著，说明抗旱能力相似。与对照相比，GLBG生长较差，但净光合速率和类胡萝卜素含量较高。本研究对大葱蜡质相关基因的鉴定和GLBG生理特性的分析，为进一步发掘大葱角质层蜡质生物合成和分泌相关基因以及在大葱育种中的潜在应用提供了参考。
The cuticle wax coating the primary aerial organs of terrestrial plants consists of epicuticular and intracuticular wax; this wax protects the plants and affects many physiological characteristics. The glossy, bright green BianGan Welsh onion (Allium fistulosumL.) (GLBG) was derived from a natural variation in the field, and it was tentatively demonstrated that the GLBG or glossy phenotype is conditioned by a single recessive gene. An analysis of the physiological characteristics related to cuticle wax showed that the density and shape of wax crystals on the leaf surface of GLBG clearly differed from those of the waxy BianGan Welsh onion (BG) and that its epicuticular wax coverage was 50% less than that of BG. However, there were no significant differences in cuticle membrane permeability or stomatal density, indicating similar drought resistance abilities. Compared to BG, GLBG had poorer growth but higher net photosynthetic rate and carotenoid content. Our research on wax-related genetic identification and the analysis of GLBG physiological characteristics provides a reference for the further exploration of genes related to cuticle wax biosynthesis and secretion in Welsh onion and for potential applications in Welsh onion breeding.