How repeatable are associations between polymorphisms in achaete-scute and bristle number variation in Drosophila?
How repeatable are associations between polymorphisms in achaete-scute and bristle number variation in Drosophila?
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DOI：
10.1534/genetics.106.067108
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发表时间：
2007-04-01
期刊：
影响因子：
3.3
通讯作者：
Long, Anthony D.
中科院分区：
文献类型：
--
作者：
Gruber, Jonathan D.;Genissel, Anne;Long, Anthony D.
目前，常见的遗传变异--特别是那些与实验室研究中的表型变异显著相关的变异--与野生条件下的表型变异之间的相关性还知之甚少。为了解决这个问题，我们在几个新的种群样本中量化了Ahaete-Scule Complex(ASC)多态与果蝇刷毛数量表型的关系。MC22是Scute转录本下游97bp的双等位非重复长度多态。先前已经证明，在一组等基因系中，它与两性胸膜刷毛数量的变异有关。我们在一大群野生捕获的黑腹果蝇中复制了这种关联。在实验室条件下保持了大约25年的近交种群中，我们也在MC22检测到了显著的关联，但这个样本中的表型效应与最初研究中估计的方向相反，最后，在第二个大型野生捕获的队列或一组134个近等基因系中没有检测到显著的关联。我们在野生样本中重复最初联系的能力表明，这不是虚假的。然而，来自其他三个小组的不一致的结果表明，多态遗传标记和导致持续变异的基因座之间的关系并不简单。
Currently, the relevance of common genetic variants - particularly those significantly associated with phenotypic variation in laboratory studies - to standing phenotypic variation in the wild is poorly understood. To address this, we quantified the relationship between achaete-scule complex (ASC) polymorphisms and Drosophila bristle number phenotypes in several new population samples. MC22 is a biallelic, non repetitive-length polymorphism 97 bp downstream of the scute transcript. It has been previously shown to be associated with sternopleural bristle number variation in both sexes in a set of isogenic lines. We replicated this association in a large cohort of wild-caught Drosophila melanogaster We also detected a significant association at MC22 in an outbred population maintained under laboratory conditions for similar to 25 years, but the phenotypic effects in this sample were opposite from the direction estimated in the initial Study, Finally, no significant associations were detected in a second large wild-caught cohort or in a set of 134 nearly isogenic lines. Our ability to repeat the initial association in wild samples suggests that it was not spurious. Nevertheless, inconsistent results from the other three panels suggest that the relationship between polymorphic genetic markers and loci contributing to continuous variation is not a simple one.